Volume 10.05 | Feb 11

ESC & iPSC News 10.05 February 11, 2015
ESC & iPSC News
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Vascular Niche Promotes Hematopoietic Multipotent Progenitor Formation from Pluripotent Stem Cells
Scientists differentiated hes2 human embryonic stem cells and Macaca nemestrina-induced pluripotent stem cell line-7 with cytokines in the presence or absence of endothelial cells that express JAG1 and DLL4. [J Clin Invest] Full Article | Press Release
Free Sample: TeSR™2 Defined, Feeder-Free and Xeno-Free Human ES and iPS Cell Medium
PUBLICATIONS (Ranked by impact factor of the journal)
Collaborative Rewiring of the Pluripotency Network by Chromatin and Signaling Modulating Pathways
Researchers show that combining ascorbic acid and MAP kinase and glycogen synthetase kinase inhibitors increases the efficiency of reprogramming from fibroblasts and synergistically enhances conversion of partially reprogrammed intermediates to the induced pluripotent stem cells state. [Nat Commun] Full Article

Rapid Reprogramming of Epigenetic and Transcriptional Profiles in Mammalian Culture Systems
The authors demonstrate that adaptation of mouse embryonic fibroblasts to cell culture results in a rapid reprogramming of epigenetic and transcriptional states. They observed global 5-hydroxymethylcytosine erasure within three days of culture initiation. [Genome Biol] Abstract | Full Article

Haploinsufficiency Screen Highlights Two Distinct Groups of Ribosomal Protein Genes Essential for Embryonic Stem Cell Fate
In a functional genomics screen of mouse embryonic stem cells with nested hemizygous chromosomal deletions, researchers reveal that ribosomal protein genes are the most significant haploinsufficient determinants for embryoid body formation. [Proc Natl Acad Sci USA] Abstract | Full Article

Ezh2 Mediated H3K27me3 Activity Facilitates Somatic Transition during Human Pluripotent Reprogramming
The authors demonstrate that histone methyl transferase activity of Ezh2 is required for mesenchymal to epithelial transition during human iPSC generation. They show that the H3K27me3 activity favors induction of pluripotency by transcriptionally targeting the TGF-β signaling pathway. [Sci Rep] Full Article

An Atypical Human Induced Pluripotent Stem Cell Line with a Complex, Stable, and Balanced Genomic Rearrangement Including a Large De Novo 1q Uniparental Disomy
Researchers report the characterization of an human induced pluripotent stem cell line generated by daily transfections of modified messenger RNAs, displaying several genomic abnormalities. Karyotype analysis showed a complex genomic rearrangement, which remained stable during long-term culture. [Stem Cells Transl Med] Abstract

DMSO Efficiently Down Regulates Pluripotency Genes in Human Embryonic Stem Cells during Definitive Endoderm Derivation and Increases the Proficiency of Hepatic Differentiation
Scientists present a novel approach to rapidly and efficiently down regulate pluripotency genes during initiation of differentiation to definitive endoderm cells by addition of dimethyl sulfoxide (DMSO) to activin A-based culture medium and report its effects on the downstream differentiation to hepatocyte-like cells. [PLoS One] Full Article

Generation of Enterocyte-Like Cells with Pharmacokinetic Functions from Human Induced Pluripotent Stem Cells Using Small-Molecule Compounds
Investigators constructed an intestinal pharmacokinetics evaluation system and to generate pharmacokinetically functional enterocytes from human induced pluripotent stem cells. [Drug Metab Dispos] Abstract

Characterization of Porcine Partially Reprogrammed iPSCs from Adipose-Derived Stem Cells
Researchers obtained partially reprogrammed induced pluripotent stem cells (iPSCs) from porcine adipose-derived stem cells by ectopic expression of human transcription factors in feeder-free condition. [Reproduction] Abstract

Use of RUNX2 Expression to Identify Osteogenic Progenitor Cells Derived from Human Embryonic Stem Cells
The authors generated a RUNX2-yellow fluorescent protein (YFP) reporter system to study osteogenic development from human embryonic stem cells (hESCs). Their studies demonstrate the fidelity of YFP expression with expression of RUNX2 and other osteogenic genes in hESC-derived osteoprogenitor cells, as well as the osteogenic specificity of YFP signal. [Stem Cell Reports] Full Article | Press Release

New TeSR™-E8™ is Here For Feeder-Free Culture of Human ES Cells and iPS Cells
Feeder Layer Cells Actions and Applications
Regenerative medicine has been gaining importance in recent years as a discipline that moves biomedical technology from the laboratory to the patients. Human stem and pluripotent cells play an important role, but feeder cells presence is necessary for these progenitor cells to grow and differentiate. The authors address recent specific applications including those associated to the growth of embryonic and induced pluripotent stem cells. [Tissue Eng Part B Rev] Abstract

Transcription Factor-Mediated Reprogramming: Epigenetics and Therapeutic Potential
The authors focus on the different ways of implementing transcription factor-mediated reprogramming, their associated epigenetic changes and its therapeutic potential. [Immunol Cell Biol] Abstract

Visit our reviews page to see a complete list of reviews in the ESC & iPSC research field.

Cell Culture World Congress 2015
Three USC Researchers Win $4.3 Million in Awards from California’s Stem Cell Agency
Three scientists from Keck Medicine of USC have won grants exceeding $4.3 million from the California Institute for Regenerative Medicine for research that includes creating a temporary liver for patients, finding novel ways to treat immune disorders and blood diseases, and developing new animal models for heart failure, diabetes and neurodegenerative diseases. [University of Southern California] Press Release

Juno Therapeutics Establishes Manufacturing Presence in Washington State
Juno Therapeutics, Inc. announced that it has entered into a lease agreement for a facility in Bothell, Washington to manufacture the company’s cell therapy products. The facility, which is expected to come online in early 2016, will support Juno’s planned JCAR015 multicenter clinical trial, additional clinical programs in Juno’s pipeline, and the company’s first commercial products. [Juno Therapeutics, Inc.] Press Release

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Moot Punishments for Japanese STAP Scientists
Four Japanese stem-cell researchers have been disciplined for their role in the scandal that gripped Japan — and the international science community — last year. The harshest punishments, however, went to two researchers who have since left their jobs. [Nature News] Editorial

NIH Proposal to Create Grant for Aging Scientists Hits a Nerve
A seemingly innocuous idea from the National Institutes of Health (NIH) for nudging aging scientists to retire is being blasted in the blogosphere. NIH’s proposal — an “emeritus” award that senior scientists would use to pass their work on to younger colleagues and wind down their labs — is unnecessary and could take funding away from younger and midcareer scientists, many commenters argued. [ScienceInsider] Editorial
NEW 2015 Conference on Tissue Engineering and Regenerative Medicine
March 18-20, 2015
Suzhou, China

Visit our events page to see a complete list of events in the ESC & iPSC community.
NEW Postdoctoral Position – Pediatric Hematology and Oncology (University Medical Center of Freiburg)

Research Associate – Pluripotent Stem Cell Biology (STEMCELL Technologies Inc.)

Scientist – Reprogramming and Pluripotent Stem Cell Biology (STEMCELL Technologies Inc.)

Scientist – Pluripotent Stem Cell Biology Endoderm Lineages (STEMCELL Technologies Inc.)

Scientist – Human Pluripotent Stem Cell Biology (STEMCELL Technologies Inc.)

Scientist – Pluripotent Stem Cell Media Development, High Throughput Screening (STEMCELL Technologies Inc.)

Scientist – Cell Culture Support Products (STEMCELL Technologies Inc.)

Research Engineer – Reproductive Stem Cell Biology (Karolinska Institute)

Graduate Research Assistantship – Reprogramming Fibroblasts into Functional Cardiomyocytes (Utah State University)

Postdoctoral Position – Human Induced Pluripotent Stem Cells (hiPSCs) and the Peripheral Nervous System (Inserm)

Senior Process Development Engineer (Sangamo BioSciences, Inc.)

Director – Vector Production (Sangamo BioSciences, Inc.)

Postdoctoral Fellowship – Stem Cell and Developmental Biology (University of Copenhagen)

Postdoctoral Positions – Human Embryonic Stem Cells and Induced Pluripotent Stem Cells (University of Macau)

PhD Studentship – Deciphering Mechanisms Underlying the Dysfunction of Motoneurons Derived from ALS Patient iPSCs (University of St. Andrews)

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