Volume 10.09 | Mar 11

ESC & iPSC News 10.09 March 11, 2015
ESC & iPSC News
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Twin Copies of a Gene Pair Up in Embryonic Stem Cells at a Critical Moment in Their Differentiation
Researchers showed that the two alleles of Oct4 did not come together randomly, at any time or place, but did so at the developmental point at which stem cells begin their maturation into specific cell types. [Press release from Cold Spring Harbor Laboratory discussing online publication in Cell Stem Cell]
Press Release | Abstract | Graphical Abstract
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PUBLICATIONS (Ranked by impact factor of the journal)
Stemness Factor Sall4 Is Required for DNA Damage Response in Embryonic Stem Cells
Scientists showed that the stemness factor Sall4 is required for activating the critical ataxia telangiectasia mutated-dependent cellular responses to DNA double-stranded breaks (DSBs) in mouse embryonic stem cells and confer their resistance to DSB-induced cytotoxicity. [J Cell Biol] Abstract | Press Release

Production of Gene-Corrected Adult Beta Globin Protein in Human Erythrocytes Differentiated from Patient iPSCs after Genome Editing of the Sickle Point Mutation
Researchers demonstrated that the CRISPR/Cas9 system designer nuclease is much more efficient in stimulating gene targeting of the endogenous HBB locus near the sickle cell disease point mutation in human induced pluripotent stem cells (iPSCs) than ZFNs and TALENs. [Stem Cells] Abstract | Press Release

Conditional-Ready Mouse Embryonic Stem Cell Derived Macrophages Enable the Study of Essential Genes in Macrophage Function
Investigators constructed a homozygous mutant mouse embryonic stem cell line in the Traf2 gene that is known to play a role in tumor necrosis factor-α signaling but has not been studied for its role in infections or response to Toll-like receptor agonists. [Sci Rep] Full Article

Human Induced Pluripotent Stem Cells Differentiate into Insulin-Producing Cells Able to Engraft In Vivo
Human induced pluripotent stem cells, derived from both fetal and adult fibroblasts, were differentiated in vitro into pancreas-committed cells and then transplanted into immunodeficient mice at two different stages of differentiation (posterior foregut and endocrine cells). [Acta Diabetol] Abstract

A Facile Method to Establish Human Induced Pluripotent Stem Cells from Adult Blood Cells under Feeder-Free and Xeno-Free Culture Conditions: A Clinically Compliant Approach
The authors report highly efficient induced pluripotent stem cell derivation under clinically compliant conditions via major improvements. They revised a combination of three EBNA1/OriP episomal vectors expressing five transgenes, which increased reprogramming efficiency by ≥10-50-fold from their previous vectors. [Stem Cells Transl Med] Abstract

Embryonic Stem Cells Derived from In Vivo or In Vitro-Generated Murine Blastocysts Display Similar Transcriptome and Differentiation Potential
Researchers generated mouse embryonic stem cells (mESC) from blastocysts conceived after natural mating or after in vitro fertilization, using optimal and suboptimal conditions. All three groups of embryos showed similar behavior during both derivation and differentiation into their respective mESC lines. [PLoS One] Full Article

The Histone Acetyltransferase Myst2 Regulates Nanog Expression, and Is Involved in Maintaining Pluripotency and Self-Renewal of Embryonic Stem Cells
Scientists showed that Myst2 plays a role in pluripotency and self-renewal of embryonic stem cells. Myst2 deficiency results in loss of characteristic morphology, decreased alkaline phosphatase staining and reduced histone acetylation, as well as aberrant expression of pluripotency and differentiation markers. [FEBS Lett] Abstract

Establishment of Tsc2-Deficient Rat Embryonic Stem Cells
To reveal the molecular basis of the pathogenesis caused by the Tsc2 mutation, investigators derived embryonic stem cells from Eker rats, which have the Tsc2 mutation and develop brain lesions and renal tumors. [Int J Oncol] Abstract

Phenotype Switching through Epigenetic Conversion
The authors recently set up a new strategy for the conversion of an adult mature cell into another cell type. They increased cell plasticity using 5-aza-cytidine and took advantage of a brief window of epigenetic instability to redirect cells to a different lineage. [Reprod Fertil Dev] Abstract

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From One-Cell to Tissue: Reprogramming, Cell Differentiation and Tissue Engineering
Inspired by the pioneering work in stem cells, particularly the remarkable reprogramming technique, the fields of regenerative medicine and tissue engineering have advanced rapidly in the past several years. Recent progress in the fields of induced pluripotent stem cells and tissue engineering is reviewed, with a focus on applications in medicine. [Bioscience] Full Article

Development of Hematopoietic Stem and Progenitor Cells from Human Pluripotent Stem Cells
The authors discuss the current status in the generation of multipotent hematopoietic stem and progenitor cells from human pluripotent stem cells via hemogenic endothelial cells. They also review the achievements in direct reprogramming from non-hematopoietic cells to hematopoietic stem and progenitor cells. [J Cell Biochem] Abstract

Visit our reviews page to see a complete list of reviews in the ESC & iPSC research field.
Athersys and Chugai Enter License Agreement and Collaboration to Develop MultiStem® Cell Therapy for Ischemic Stroke in Japan
Athersys, Inc. and Chugai Pharmaceutical Co., Ltd. have announced a partnership and license agreement to exclusively develop and commercialize MultiStem® cell therapy for ischemic stroke in Japan. [Athersys, Inc.] Press Release

Orgenesis Acquires Cell Therapy Manufacturer MaSTherCell
Orgenesis Inc. announced the company’s plan to increase its global presence and capabilities by acquiring MaSTherCell, an emerging pioneer in the industrialization of cell-based therapeutics. [Orgenesis Inc. (Marketwire L.P.)] Press Release

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In Japan, Embattled RIKEN Chief to Step Down
Ryoji Noyori plans to resign as president of RIKEN, the network of Japanese national labs that has spent much of the past year embroiled in a fraud scandal, news outlets report. [ScienceInsider] Editorial

Australian Research Facilities under Threat
Much of Australia’s shared national research infrastructure is under threat of closure because it is not clear whether it will get the $150 million allocated in its 2015–16 budget. The cash is tied to the passage of controversial higher-education-reform legislation, which has been held up in parliament. [Nature News] Editorial

Europe’s Human Brain Project Needs Urgent Reforms, Panel Says
The Human Brain Project (HBP), a humongous, controversial research project backed by the European Union, must reform to stay on course, a review panel has recommended and it must do so fast. A summary of the panel’s report, published by the European Commission, says a series of “corrective actions” needs to be taken in HBP’s governance, the way it collaborates, and its communication. [ScienceInsider] Editorial

NEW Mouse Development, Stem Cells & Cancer
June 4-24, 2015
Cold Spring Harbor, United States

NEW 2015 World Stem Cell Summit (WSCS15)
December 10-12, 2015
Atlanta, United States

Visit our events page to see a complete list of events in the ESC & iPSC community.
NEW Postdoctoral Associate – RNA Modification in Stem Cell Biology and Cancer Development (Sanford-Burnham Medical Research Institute)

NEW Postdoctoral Positions – Interface of Physics and Stem Cell Biology (University of Copenhagen)

Scientist – Reprogramming and Pluripotent Stem Cell Biology (STEMCELL Technologies Inc.)

Scientist – Pluripotent Stem Cell Biology Endoderm Lineages (STEMCELL Technologies Inc.)

Scientist – Pluripotent Stem Cell Media Development, High Throughput Screening (STEMCELL Technologies Inc.)

Postdoctoral Research Associate – Stem Cell Engineering (Rensselaer Polytechnic Institute)

Research Associate – Stem Cells and Regenerative Medicine (King’s College London)

Principal Investigators – Cell & Developmental Biology (University of Dundee)

Postdoctoral Position – Human Induced Pluripotent Stem Cells (hiPSCs) and the Peripheral Nervous System (Inserm)

Senior Process Development Engineer (Sangamo BioSciences, Inc.)

Director – Vector Production (Sangamo BioSciences, Inc.)

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