Volume 10.17 | May 6

ESC & iPSC News 10.17 May 6, 2015
ESC & iPSC News
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Odd Histone Helps Suppress Jumping Genes in Stem Cells
A family of proteins known as histones provides support and structure to DNA, but for years, scientists have been puzzling over occasional outliers among these histones, which appear to exist for specific, but often mysterious reasons. Now, researchers have uncovered a new purpose for one such histone variant: preventing genetic mutations by keeping certain so-called “jumping genes” in place. [Press release from Rockefeller University discussing online prepublication in Nature] Press Release | Abstract
Free Wallchart: Small Molecules, Big Impact in PSC Research
PUBLICATIONS (Ranked by impact factor of the journal)
Filia Is an ESC-Specific Regulator of DNA Damage Response and Safeguards Genomic Stability
The authors described the identification of Filia as a specific regulator of genomic stability in mouse embryonic stem cells (ESCs). [Cell Stem Cell] Abstract | Graphical Abstract

Tex10 Coordinates Epigenetic Control of Super-Enhancer Activity in Pluripotency and Reprogramming
Scientists dissected the molecular control mechanism of super enhancer activity in pluripotency and reprogramming. Starting from a protein interaction network surrounding Sox2, they identified Tex10 as a key pluripotency factor that plays a functionally significant role in embryonic stem cell self-renewal, early embryo development, and reprogramming. [Cell Stem Cell] Abstract | Graphical Abstract

SMADs and YAP Compete to Control Elongation of β-Catenin: LEF-1-Recruited RNAPII during hESC Differentiation
Researchers found that LEF-1 and other human embryonic stem cells (hESC) enhancers recruited RNAPII complexes that are highly phosphorylated at Ser5, but not Ser7. [Mol Cell] Abstract | Graphical Abstract

TET1 Is Controlled by Pluripotency-Associated Factors in ESCs and Downmodulated by PRC2 in Differentiated Cells and Tissues
Investigators showed that the Tet1 gene is regulated, both in mouse and human embryonic stem cells (ESCs), by the stemness specific factors Oct3/4, Nanog and by Myc. [Nucleic Acids Res] Abstract

An HDAC2-TET1 Switch at Distinct Chromatin Regions Significantly Promotes the Maturation of Pre-iPS to iPS Cells
The authors report that knockdown of histone deacetylase 2 (HDAC2) specifically promoted the maturation of induced pluripotent (iPS) stem cells. [Nucleic Acids Res] Full Article

Efficient CRISPR-Cas9-Mediated Generation of Knockin Human Pluripotent Stem Cells Lacking Undesired Mutations at the Targeted Locus
Investigators systematically tested the ability of CRISPR-Cas9 to mediate reporter gene knockin at 16 distinct genomic sites in human pluripotent stem cells. [Cell Rep] Full Article | Graphical Abstract

Systematic Optimization of Human Pluripotent Stem Cells Media Using Design of Experiments
Scientists applied design of experiments, a powerful statistical tool, to improve the medium formulation for hPSC. Using pluripotency and cell growth as read-outs, they determined the optimal concentration of both basic fibroblast growth factor and neuregulin−1 beta 1. [Sci Rep] Full Article | Press Release

Small-Molecule-Driven Hepatocyte Differentiation of Human Pluripotent Stem Cells
Researchers devised a growth-factor-free protocol that relies on small molecules to differentiate human pluripotent stem cells toward a hepatic phenotype. [Stem Cell Reports] Full Article

High Glutathione and Glutathione Peroxidase-2 Levels Mediate Cell-Type-Specific DNA Damage Protection in Human Induced Pluripotent Stem Cells
In human induced pluripotent stem cells, the authors found that genome surveillance is achieved via two ways, namely, a hypersensitivity to apoptosis and a very low accumulation of DNA lesions. [Stem Cell Reports] Full Article | Graphical Abstract

Learn more about our cell attachment substrates and matrices for pluripotent stem cell research.
De Novo Generation of HSCs from Somatic and Pluripotent Stem Cell Sources
The authors discuss the latest advancements in hematopoietic stem cell (HSC) generation by directed differentiation from human pluripotent stem cells or direct conversion from somatic cells, and highlight their applications in research and prospects for therapy. [Blood] Abstract

Dendritic Cells and Pluripotency: Unlikely Allies in the Pursuit of Immunotherapy
The authors discuss how the directed differentiation of human pluripotent stem cells may address many of the current obstacles to the use of monocyte-derived dendritic cells (DC) in immunotherapy, providing a novel source of previously inaccessible dendritic cell subsets and opportunities for their scale-up, quality control and genetic modification. [Regen Med] Abstract

Visit our reviews page to see a complete list of reviews in the ESC & iPSC research field.
ViaCyte to Present at GTCbio 2015 Diabetes Summit
ViaCyte, Inc. announced two presentations at GTCbio 2015 Diabetes Summit in Boston. Dr. D’Amour presented on the VC-01™ product candidate for the treatment of type 1 diabetes, which recently entered the clinical phase of development, and Dr. Agulnick discussed results in animal models utilizing insulin-positive cells generated in vitro from pluripotent stem cells. [Press release from ViaCyte, Inc. discussing research presented at the GTCbio 2015 Diabetes Summit, Boston] Press Release

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STEMCELL Technologies to Commercialize BrainPhys™ Neuronal Culture Medium for Improved Support of Neurophysiological Activity
STEMCELL Technologies Inc. has announced that it has signed a license agreement with the Salk Institute for Biological Sciences for the rights to commercialize BrainPhys™ Neuronal Medium. [STEMCELL Technologies Inc. (Business Wire)] Press Release

£2 Million for Edinburgh Multiple Sclerosis Research
The Mulitple Sclerosis (MS) Society announced it is investing a further £2 million into the Edinburgh Centre for MS Research. The Edinburgh Centre investigators will focus their research on stem cells, in the hope of building a clearer picture of how MS develops and a better method for modeling the condition and finding effective treatments. [University of Edinburgh] Press Release
New Version of Cures Bill Recommends $10 Billion Boost for NIH
An ambitious effort in the U.S. House of Representatives to jump-start biomedical innovation took another step forward with the release of a bipartisan draft bill. The so-called 21st Century Cures Act contains huge news for supporters of biomedical research: It recommends substantial budget increases for the National Institutes of Health (NIH), including $10 billion in extra funding over five years. [ScienceInsider] Editorial

NIH Reiterates Ban on Editing Human Embryo DNA
The US National Institutes of Health (NIH) has reaffirmed its ban on research that involves gene editing of human embryos. In a statement, NIH director Francis Collins spelled out the agency’s long-standing policy against funding such research and the ethical and legal reasons for it. [Nature News] Editorial
NEW Nordic Life Science Days 2015
September 9-10, 2015
Stockholm, Sweden

NEW Frontiers in Stem Cells and Regeneration
September 27-October 3, 2015
Woods Hole, United States

Visit our events page to see a complete list of events in the ESC & iPSC community.
NEW Scientist – Human Pluripotent Stem Cell Biology (STEMCELL Technologies Inc.)

NEW Postdoctoral Position – Cell Replacement Therapy for Parkinson’s Disease (Memorial Sloan Kettering Cancer Center )

NEW Postdoctoral Research Fellow – Stem Cell Disease Modeling (Nanyang Technological University)

Postdoctoral Position – Stem Cell Biology, Epigenetics & Cancer (Albert Einstein College of Medicine of Yeshiva University)

Postdoctoral Fellow – Epithelial Stem Cells & Membrane Trafficking (New York University School of Medicine)

Postdoctoral Fellowship – Stem Cells & Epigenetics (National Institutes of Health)

Stem Cell Culture Scientist (Paramount Recruitment)

Postdoctoral Fellowship – Stem Cells & Epigenetics (National Institutes of Health)

Postdoctoral Position – Induced Pluripotent Stem Cells to Study Metabolism and Gene Expression Regulating Neural Tube Development (Joslin Diabetes Center)

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