Volume 10.30 | Aug 12

ESC & iPSC News 10.30 August 12, 2015
ESC & iPSC News
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TDP-43 Repression of Nonconserved Cryptic Exons Is Compromised in ALS-FTD
Investigators found that TDP-43 repressed the splicing of nonconserved cryptic exons, maintaining intron integrity. When TDP-43 was depleted from mouse embryonic stem cells, these cryptic exons were spliced into messenger RNAs, often disrupting their translation and promoting nonsense-mediated decay. [Science] Abstract | Press Release
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PUBLICATIONS (Ranked by impact factor of the journal)
Excessive Cellular Proliferation Negatively Impacts Reprogramming Efficiency of Human Fibroblasts
Scientists report that rapid proliferation of human somatic fibroblasts is detrimental to reprogramming efficiency when reprogrammed using a lentiviral vector expressing OCT4, SOX2, KLF4, and cMYC in insulin-rich defined medium. [Stem Cells Transl Med] Abstract

Human iPSC Derived Disease Model of MERTK-Associated Retinitis Pigmentosa
The authors report successful generation of human induced pluripotent stem (iPSC) cells from skin fibroblasts of a patient harboring a novel Ser331Cysfs*5 mutation in the MERTK gene. [Sci Rep] Full Article

Influence of Oriented Nanofibrous PCL Scaffolds on Quantitative Gene Expression during Neural Differentiation of Mouse Embryonic Stem Cells
Embryoid bodies, which contained all three embryonic germ layers, were cultured on poly Ɛ-caprolactone (PCL) scaffolds to study the effect of fiber orientation on cell morphology and differentiation. [J Biomed Mater Res A] Abstract

Dysregulation of the Transforming Growth Factor β Pathway in Induced Pluripotent Stem Cells Generated from Patients with Diamond Blackfan Anemia
Scientists generated induced pluripotent stem cells from diamond blackfan anemia (DBA) patients carrying RPS19 or RPL5 mutations. Transcriptome analysis revealed the striking dysregulation of the transforming growth factor β signaling pathway in DBA lines. [PLoS One] Full Article

Scriptaid Treatment Decreases DNA Methyltransferase 1 Expression by Induction of MicroRNA-152 Expression in Porcine Somatic Cell Nuclear Transfer Embryos
Researchers investigated the effects of Scriptaid on the development of porcine somatic cell nuclear transfer embryos in vitro and its mechanism. [PLoS One] Full Article

Metabolic Suppression during Mesodermal Differentiation of Embryonic Stem Cells Identified by Single-Cell Comprehensive Gene Expression Analysis
Researchers separated Flk-1+ and Flk-1 cells from spontaneously differentiating embryoid bodies of mouse embryonic stem cells. They found that cell aggregates derived from late stage Flk-1+ cells had a relatively small size and a low oxygen consumption rate compared with those derived from Flk-1 cells. [Mol Biosyst] Abstract | Graphical Abstract

Kinin-B1 and B2 Receptor Activity in Proliferation and Neural Phenotype Determination of Mouse Embryonic Stem Cells
Investigators showed that kinin-B1 and B2 receptors are differentially expressed in mouse embryonic E14Tg2A stem cells undergoing neural differentiation. [Cytometry A] Abstract

Conditionally Replicating Adenovirus Prevents Pluripotent Stem Cell–Derived Teratoma by Specifically Eliminating Undifferentiated Cells
Scientists demonstrated that conditionally replicating adenoviruses that specifically target cancers using multiple factors, originally developed as anticancer drugs, may also be useful as novel antitumorigenic agents in human pluripotent stem cell-based therapy. [Mol Ther Methods Clin Dev] Full Article

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In Vitro T-Cell Generation from Adult, Embryonic, and Induced Pluripotent Stem Cells: Many Roads to One Destination
The authors discuss current methods for the in vitro derivation of T cells from murine and human hematopoietic stem/progenitor cells and human pluripotent stem cells that use feeder-cell and feeder-cell-free systems. They explore their potential for adoption for use in T-cell-based therapies. [Stem Cells] Abstract

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BioTime Announces the Issuance of 27 New Patents Protecting Stem Cell-Based Product Development
BioTime, Inc. announced that 27 new patents have been issued during the first half of 2015 that are owned or licensed to BioTime or its subsidiaries. These new patents add to the portfolio of over 700 issued and pending patents and patent applications world wide that are owned or licensed to BioTime or its subsidiaries. [Biotime, Inc.] Press Release

Researchers Win Contract to Study Stem Cells from Early-Onset Alzheimer’s Disease Patients
Miller School researchers have won a three-year, $721,423 U.S. Department of Defense contract to ascertain and analyze induced pluripotent stem cells from early-onset Alzheimer’s Disease patients with specific Alzheimer’s-linked genetic mutations. [University of Miami, Miller School of Medicine] Press Release

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Obama Announces Nominees for NSF Deputy Director, Head of DOE Office of Science
The White House announced that President Barack Obama has nominated engineer Richard Buckius, currently the National Science Foundation’s (NSF’s) chief operating officer, to fill the agency’s second highest position: deputy director. [ScienceInsider] Editorial

Caution Urged over Editing DNA in Wildlife (Intentionally or Not)
The US National Academy of Sciences, Engineering, and Medicine held the first in a series of meetings meant to find ways to balance the promise and perils of the ‘gene drive’ technique. The method can rapidly modify not just a single organism but a whole population, by inserting a desired genetic modification into an organism along with DNA that increases the rate at which the change is passed to the next generation. [Nature News] Editorial
NEW Keystone Symposia – Stem Cells and Regeneration in the Digestive Organs
March 13-17, 2016
Keystone, United States

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NEW Senior Research Technician – Cancer and Inflammatory Bowel Disease (Qu Biologics)

Scientist – Human Pluripotent Stem Cell Biology (STEMCELL Technologies Inc.)

Advanced Research Assistant – Gene Targeting (Wellcome Trust Sanger Institute)

Head of Research – Cellular Operations (Welcome Trust Sanger Institute)

Postdoctoral Research Fellow – Epigenetics (Institute of Molecular Biology Mainz)

Postdoctoral Position – Stem Cell Biophysics (University Paris Diderot)

Research Fellow – Tissue Engineering and Regenerative Medicine (National Cancer Center Singapore)

Postdoctoral Positions – Human ES & iPS Cells and Heart Regenerative Medicine (University of Pittsburgh)

MICA Stem Cell Scientist (Medicines and Healthcare Products Regulatory Agency)

Stem Cell Scientist (Medicines and Healthcare Products Regulatory Agency)

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