Volume 10.32 | Aug 26

ESC & iPSC News 10.32 August 26, 2015
ESC & iPSC News
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Limiting Replication Stress during Somatic Cell Reprogramming Reduces Genomic Instability in Induced Pluripotent Stem Cells
Researchers showed that, similar to the phenomenon of oncogene-induced replication stress, the expression of reprogramming factors induces replication stress. Increasing the levels of the checkpoint kinase 1 reduced reprogramming-induced replication stress and increased the efficiency of induced pluripotent stem cells generation. [Nat Commun] Full Article
Free Wallchart from Nature Reviews Neuroscience on Modeling Neurological Disease with iPS Cells
PUBLICATIONS (Ranked by impact factor of the journal)
PRC2 Is Required to Maintain Expression of the Maternal Gtl2-Rian-Mirg Locus by Preventing De Novo DNA Methylation in Mouse Embryonic Stem Cells
Scientists showed that polycomb repressive complex 2 (PRC2) is required to maintain expression of maternal microRNAs and long non-coding RNAs from the Gtl2-Rian-Mirg locus, which is essential for full pluripotency of induced pluripotent stem cells. [Cell Rep] Full Article | Graphical Abstract

Functional Gene Correction for Cystic Fibrosis in Lung Epithelial Cells Generated from Patient iPSCs
Investigators generated induced pluripotent stem cells (iPSCs) from cystic fibrosis patients carrying a homozygous deletion of F508 in the cystic fibrosis transmembrane regulator gene (CFTR), which resulted in defective processing of CFTR to the cell membrane. [Cell Rep] Full Article | Graphical Abstract

Ube2s Regulates Sox2 Stability and Mouse ES Cell Maintenance
Scientists identified ubiquitin-conjugating enzyme E2S (Ube2s) as a novel effector for Sox2 protein degradation. Ube2s mediated K11-linked polyubiquitin chain formation at the Sox2-K123 residue, thus marking it for proteasome-mediated degradation. [Cell Death Differ] Abstract

Bcl3 Bridges LIF-STAT3 to Oct4 Signaling in the Maintenance of Naïve Pluripotency
Scientists verified B cell leukemia/lymphoma 3 (Bcl3) as the most significantly downregulated factor following leukemia inhibitory factor (LIF) withdrawal in mouse embryonic stem cells (mESCs). Bcl3 knockdown altered mESC morphology, reduced expression of pluripotency genes including Oct4, Sox2 and Nanog, and downregulated DNA binding of acetylated histone 3 and RNA polymerase II on the Oct4 promoter. [Stem Cells] Abstract

Heterogeneous Lineage Marker Expression in Naive Embryonic Stem Cells Is Mostly Due to Spontaneous Differentiation
Researchers showed the transcriptome of Nanog-negative cells exhibited expression of classes of genes associated with differentiation that are not yet active in cells exposed to differentiation conditions for one day. [Sci Rep] Full Article

Glycosyltransferase ST6GAL1 Contributes to the Regulation of Pluripotency in Human Pluripotent Stem Cells
Investigators observed that the sialyltransferase ST6GAL1 was preferentially expressed in undifferentiated human pluripotent stem cells compared to non-pluripotent cells. [Sci Rep] Full Article

Reprogramming Roadblocks Are System Dependent
Scientists demonstrated that reprogramming triggered by less efficient polycistronic reprogramming cassettes not only highlights mesenchymal-to-epithelial transition (MET) as a roadblock but also faces more severe difficulties to attain a pluripotent state even post-MET. [Stem Cell Rep] Full Article | Graphical Abstract

Nucleosome Organization in Human Embryonic Stem Cells
Researchers revealed that the genome is a determinant of nucleosome organization with transcriptionally inactive regions characterized by a “ground state” of nucleosome profiles driven by underlying DNA sequences. [PLoS One] Full Article

Uniparental Disomy of the Entire X Chromosome in Turner Syndrome Patient-Specific Induced Pluripotent Stem Cells
Scientists generated induced pluripotent stem cells from four 45,X chorionic villous fibroblast lines and found that only one reprogrammed line acquired 46,XX karyotype via uniparental disomy of the entire X chromosome. [Cell Disc] Full Article

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Induced Pluripotent Stem Cells: A New Frontier for Stem Cells in Dentistry
The authors summarize the dental tissues that have successfully been utilized to generate induced pluripotent stem cell (iPSC) lines, as well as the potential uses of iPSCs for tissue regeneration in different dental applications. [J Dent Res] Abstract

Visit our reviews page to see a complete list of reviews in the ESC & iPSC research field.

IBC’s Cell Therapy Bioprocessing & Commercialization meeting
STEMCELL Technologies Inc. Enters a Licensing Agreement with iPS Academia Japan, Inc. for Induced Pluripotent Stem Cell Research Technologies
STEMCELL Technologies Inc. has signed a patent license agreement with iPS Academia Japan, Inc. to license and commercialize iPS Academia Japan, Inc.’s patented technologies for induced pluripotent stem cell (iPS cell) research. [STEMCELL Technologies Inc. (Business Wire)] Press Release

$52M NIH Grant Advances Clinical and Translational Research at UC San Diego
The Clinical and Translational Research Institute at University of California, San Diego has received a five-year Clinical and Translational Science Award for approximately $52 million from the National Center for Advancing Translational Science, part of the National Institutes of Health (NIH). [UC San Diego Health] Press Release

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China Announces Stem-Cell Rules
Chinese stem-cell scientists have welcomed long-awaited measures that, state media claim, will rein in rogue use of stem cells in clinics while allowing research. [Nature News] Editorial

Faked Peer Reviews Prompt 64 Retractions
A leading scientific publisher has retracted 64 articles in ten journals, after an internal investigation discovered fabricated peer-review reports linked to the articles’ publication. Berlin-based Springer announced the retractions in a statement. [Nature News] Editorial
NEW 3rd Annual Cell Culture & Bioprocessing Asia Congress 2015
October 15-16, 2015
Singapore, Singapore

Visit our events page to see a complete list of events in the ESC & iPSC community.
NEW Postdoctoral Research Fellow – Development and Pathogenesis of Pancreatic Cancer (University College London)

Scientist – Human Pluripotent Stem Cell Biology (STEMCELL Technologies Inc.)

Postdoctoral Fellow – (Johns Hopkins University School of Medicine)

Senior Research Technician – Cancer and Inflammatory Bowel Disease (Qu Biologics)

Advanced Research Assistant – Gene Targeting (Wellcome Trust Sanger Institute)

Head of Research – Cellular Operations (Welcome Trust Sanger Institute)

Postdoctoral Research Fellow – Epigenetics (Institute of Molecular Biology Mainz)

Postdoctoral Position – Stem Cell Biophysics (University Paris Diderot)

MICA Stem Cell Scientist (Medicines and Healthcare Products Regulatory Agency)

Stem Cell Scientist (Medicines and Healthcare Products Regulatory Agency)

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