Volume 10.41 | Oct 28

ESC & iPSC News 10.41 October 28, 2015
ESC & iPSC News
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A Comparison of Genetically Matched Cell Lines Reveals the Equivalence of Human iPSCs and ESCs
Researchers used genetically matched human embryonic stem cell (ESC) and human induced pluripotent stem cell (iPSC) lines to assess the contribution of cellular origin, the Sendai virus reprogramming method and genetic background to transcriptional and DNA methylation patterns while controlling for cell line clonality and sex. [Nat Biotechnol] Abstract
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PUBLICATIONS (Ranked by impact factor of the journal)
A qPCR ScoreCard Quantifies the Differentiation Potential of Human Pluripotent Stem Cells
The authors report an improved version of the assay based on qPCR that enables faster, more quantitative assessment of functional pluripotency. They provided an in-depth characterization of the revised signature panel through embryoid body and directed differentiation experiments as well as a detailed comparison to the teratoma assay. [Nat Biotechnol] Abstract

Regeneration of Thyroid Function by Transplantation of Differentiated Pluripotent Stem Cells
Scientists used mouse pluripotent stem cells to identify key conserved roles for BMP and FGF signaling in regulating thyroid lineage specification from foregut endoderm in mouse and Xenopus. [Cell Stem Cell]
Abstract | Graphical Abstract | Press Release

Atg5-Independent Autophagy Regulates Mitochondrial Clearance and Is Essential for iPSC Reprogramming
Scientists suggest that an Atg5-independent autophagic process mediates mitochondrial clearance, a characteristic event involved in the metabolic switch. They found that blocking such autophagy, but not canonical autophagy, inhibits mitochondrial clearance, in turn, preventing iPSC induction. [Nat Cell Biol] Abstract

Reprogramming Human B-Cells into Induced Pluripotent Stem Cells and Its Enhancement by C/EBPα
The authors showed for the first time that freshly isolated non-cultured human cord blood- and peripheral blood-derived CD19+CD20+ B-cells can be reprogrammed to induced pluripotent stem cells carrying complete VDJH immunoglobulin gene monoclonal rearrangements using non-integrative tetracistronic, but not monocistronic, Oct4, Sox2, Klf4 and Myc-expressing Sendai Virus. [Leukemia] Abstract

Absence of WASp Enhances Hematopoietic and Megakaryocytic Differentiation in a Human Embryonic Stem Cell Model
Investigators used zinc finger nucleases targeted to the Wiskott-Aldrich syndrome (WAS) locus for the development of two isogenic WAS knockout (WASKO) human embryonic stem cell lines (hESCs). Upon hematopoietic differentiation, hESCs-WASKO generated increased ratios of CD34+CD45+ progenitors with altered responses to stem cell factor compared to hESCs-WT. [Mol Ther] Abstract | Full Article

Control of Immune Response to Allogeneic Embryonic Stem Cells by CD3 Antibody–Mediated Operational Tolerance Induction
The authors established in autoimmunity and in transplantation that CD3 antibody therapy induces a state of immune tolerance. Promising results have been obtained with CD3 antibodies in the clinic. In this study, they tested whether this strategy can prolong the survival of undifferentiated embryonic stem cells and their differentiated derivatives in histoincompatible hosts. [Am J Transplant] Abstract

Gingival Fibroblasts as Autologous Feeders for Induced Pluripotent Stem Cells
Researchers established an autologous human gingival fibroblast-derived induced pluripotent stem cell (hGF-iPSC) culture system by evaluating the feeder ability of hGFs. In both serum-containing and serum-free media, hGFs showed higher proliferation than human dermal fibroblasts. [J Dent Res] Abstract

Sox7 Is Dispensable for Primitive Endoderm Differentiation from Mouse ES Cells
The authors characterized the function of Sox7 in primitive endoderm differentiation using mouse embryonic stem (ES) cells as a model system. They showed that ectopic expression of Sox7 in ES cells has a marginal effect on triggering differentiation into primitive endoderm-like cells. [BMC Dev Biol] Full Article

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Vascular Disease Modeling Using Induced Pluripotent Stem Cells: Focus in Hutchinson-Gilford Progeria Syndrome
Several lines of induced pluripotent stem cells have been generated in the last seven years that changed the paradigm for studying diseases and the discovery of new drugs to treat them. The authors focus their attention to vascular diseases in particular Hutchinson-Gilford Progeria Syndrome, a devastating premature aging disease caused by a mutation in the lamin A gene. [Biochem Biophys Res Commun] Abstract

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New York Stem Cell Foundation Announces $7.5 Million to Five New NYSCF — Robertson Investigators
The New York Stem Cell Foundation (NYSCF) announced the 2015 class of NYSCF – Robertson Investigators, selecting five of the most talented scientists. Each investigator will receive a $1.5 million award, disbursed over the next five years. [The New York Stem Cell Foundation (EurekAlert!)] Press Release

Bogus Cell Therapy Market Worth Up to $2.4 Billion
The global market for unproven cell therapies may be worth as much as $2.4 billion, according to a report published by the International Society for Cellular Therapy. [Thomson Reuters] Press Release

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Out of Regulatory Limbo, 23andMe Resumes Some Health Tests and Hopes to Offer More
As DNA-testing firm 23andMe resumes selling health-related services in the wake of a regulatory shutdown, the company says that it remains committed to its original business plan of providing genetic information directly to consumers. [Nature News] Editorial

Canada’s New Government May Be a New Beginning for Science
Canadian scientists have been making do for nearly a decade, in the face of funding cuts, federal lab closures, and a blanket gag order on government researchers. For them, the fall of Prime Minister Stephen Harper’s Conservative government in the national election wasn’t just a relief—it was the rough emotional equivalent of blowing up the Death Star. [Wired] Editorial
NEW Stem Cells and Organoids as Models of Tissue Differentiation and Disease
January 12, 2016
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NEW Team Leader Position – Developmental Biology (Institut de Biologie Paris-Seine)

Postdoctoral Position – Neuroscience/Stem Cell Research (University of California, Davis)

Postdoctoral Position – Tissue Engineering and Regenerative Medicine (Northwestern University)

Postdoctoral Fellow – iPSC Research (Icahn School of Medicine at Mount Sinai)

Postdoctoral Position – Biomaterials for Cell Reprogramming (Institute of Tissue Regeneration Engineering)

Postdoctoral Position – Stem Cells, Development, and Cancer Biology (University of California, Santa Cruz)

Assistant/Associate/Full Professor – Biomedical Engineering (Worcester Polytechnic Institute)

Assistant Professor – Stem Cell Biology (University of South Carolina)

Postdoctoral Position – Spinal Cord Injury Research (Indiana University-Purdue University)

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