Volume 7.00 | Jan 4

ESC & iPSC News 7.00 January 4, 2012
ESC & iPSC News
     In this issue: Publications | Industry News | Policy News | Events | Jobs
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TOP STORY
Mbd3/NURD Complex Regulates Expression of 5-Hydroxymethylcytosine Marked Genes in Embryonic Stem Cells
Here, scientists examine the roles of several chromatin regulators whose loss affects the pluripotent state of embryonic stem cells. [Cell] Abstract

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PUBLICATIONS (Ranked by Impact Factor of the Journal)
A Poised Chromatin Platform for TGF-β Access to Master Regulators
Researchers report that nodal TGF-β signals use the poised histone mark H3K9me3 to trigger differentiation of mammalian embryonic stem cells. [Cell] Abstract

Human Long Non-Coding RNAs Promote Pluripotency and Neuronal Differentiation by Association with Chromatin Modifiers and Transcription Factors
This study indicates that long non-coding RNAs (lncRNAs) are important regulators of pluripotency and neurogenesis, and represents important evidence for an indispensable role of lncRNAs in human brain development. [EMBO J] Abstract

Pluripotency Factor-Mediated Expression of the Leptin Receptor Links Obesity to Oncogenesis through Tumor-Initiating Stem Cells
Researchers demonstrate that robust and selective expression of the receptor for the adipocyte-derived peptide hormone leptin is a characteristic feature of tumor-initiating stem cells and of a broad array of embryonic and induced pluripotent stem cells and is mediated directly by the core pluripotency-associated transcription factors OCT4 and SOX2. [Proc Natl Acad Sci USA] Abstract

Activin and BMP4 Synergistically Promote Formation of Definitive Endoderm in Human Embryonic Stem Cells
Scientists show that combining a second related ligand, BMP4, in combination with Activin A yields 15 to 20% more definitive endoderm as compared to Activin A alone. [Stem Cells] Abstract

Thermal Stability of FGF Protein Is a Determinant Factor in Regulating Self-Renewal, Differentiation and Reprogramming in Human Pluripotent Stem Cells
Here researchers show that only a few FGF family members (FGF2, FGF4, FGF6 and FGF9) are able to sustain strong ERK phosphorylation and NANOG expression levels in human ES cells. [Stem Cells] Abstract

Protein Kinase C Mediated Extraembryonic Endoderm Differentiation of Human Embryonic Stem Cells
Here investigators show that phorbol ester 12-O-Tetradecanoylphorbol 13-acetate causes human embryonic stem (ES) cells to undergo an epithelial mesenchymal transition and to differentiate into cells expressing markers of parietal endoderm, another extraembryonic lineage. They further confirmed that this differentiation is through the activation of PKC pathway and demonstrated that a particular PKC subtype, PKC-delta, is most responsible for this transition. [Stem Cells] Abstract

Differentiation Efficiency of Induced Pluripotent Stem Cells Depends on the Number of Reprogramming Factors
In order to elucidate the influence of factor reduction on subsequent differentiation, scientists compared the efficiency of neuronal differentiation in induced pluripotent stem cells (iPSC) generated from postnatal murine neural stem cells with either one (Oct4; iPSC1F-NSC), two (Oct4, Klf4; iPSC2F-NSC) or all four factors (iPSC4F-NSC) with those of embryonic stem cells and iPSC produced from fibroblasts with all four factors (iPSC4F-MEF). [Stem Cells] Abstract

Yin Yang 1 Extends the Myc-Related Transcription Factors Network in Embryonic Stem Cells
Researchers show that, in mouse embryonic stem cells, Yin Yang 1 has genome-wide Polycomb group protein-independent activities while it is still stably associated with the INO80 chromatin-remodeling complex, as well as with novel RNA helicase activities. [Nucleic Acids Res] Abstract

Acute Depletion of Tet1-Dependent 5-Hydroxymethylcytosine Levels Impairs LIF/Stat3 Signaling and Results in Loss of Embryonic Stem Cell Identity
Here investigators demonstrate that, unlike genetic deletion, RNAi-mediated depletion of Tet1 in mouse embryonic stem cells (mESCs) led to a significant reduction in 5-hydroxymethylcytosine and loss of mESC identity. [Nucleic Acids Res] Abstract

Naive and Primed Murine Pluripotent Stem Cells Have Distinct miRNA Expression Profiles
Here, scientists performed deep sequencing to profile microRNA (miRNA) expression in naive (embryonic stem cell) and primed (epiblast stem cell) pluripotent stem cells derived from mouse embryos of identical genetic background. [RNA] Abstract

Use of UTF1 Genetic Control Elements as iPSC Reporter
Here, researchers generated a reliable reporter system that was used to monitor induced pluripotent stem cell (iPSC) formation and subsequent differentiation. [Stem Cell Rev] Abstract

Derivation of Novel Genetically Diverse Human Embryonic Stem Cell Lines
Researchers have derived twelve new human embryonic stem cell lines from discarded, genetically normal and abnormal embryos using the latest techniques. [Stem Cells Dev] Abstract

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INDUSTRY NEWS
Kyoto Research Team to Create Stem Cell Bank
A research team has announced its plans to create a stem cells bank that will preserve artificially created stem cells to be used in regenerative medicine, it has been revealed. [The Mainichi Daily News]
Press Release

New Source of Stem Cell Generation Pioneered at UCF
A process that prompts a single gene to generate millions of supercharged stem cells has been patented at the University of Central Florida (UCF). [University of Central Florida] Press Release
POLICY NEWS
NIH Establishes National Center for Advancing Translational Sciences
In a move to re-engineer the process of translating scientific discoveries into new drugs, diagnostics, and devices, the National Institutes of Health (NIH) has established the National Center for Advancing Translational Sciences. [National Institutes of Health, United States] Press Release

Spain Cuts Science Ministry in Government Changeover
Spain’s ministry of science is the latest casualty of changes to the country’s government. Mariano Rajoy, leader of the newly elected right-wing People’s Party that took over, pledged to reduce the number of ministries from 15 to 12 to save money and improve governance [Ministry of Science, Spain] Press Release

Singh Promises Windfall for India’s Scientists
India’s Prime Minister Manmohan Singh has vowed to more than double his nation’s spending on R&D over the next 5 years and build two major research facilities. [India’s Prime Minister, India] Press Release

Guidance for Industry – Current Good Tissue Practice (CGTP) and Additional Requirements for Manufacturers of Human Cells, Tissues, and Cellular and Tissue-Based Products (HCT/Ps) [Center for Biologics Evaluation and Research, United States]
EVENTS
NEW 8th Annual Swiss Stem Cell Network Meeting
February 10, 2012
Zurich, Switzerland

NEW Stem Cells USA & Regenerative Medicine Congress
September 18-20, 2012
Boston, United States

Visit
our events page to see a complete list of events in the ESC & iPSC community.
JOB OPPORTUNITIES

Research and Development Technologist, hPSC Media (STEMCELL Technologies)

Scientist – Pluripotent Stem Cells (STEMCELL Technologies)

Research Technologist, Manufacturing Sciences (STEMCELL Technologies)

Business Analyst – Product Management (STEMSOFT Software)

Postdoctoral/Research Associate (Children’s Memorial Research Center)

Postdoctoral Position (University of California, Davis)

PhD Research Fellowship, Centre for Molecular Medicine (University of Oslo)

Junior Research Fellow (Christian Medical College)

Postdoctoral Positions (National Centre for Biological Sciences)

Scientist – Differentiation Segment (Stemgent, Inc.)


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