Volume 8.00 | Jan 2

ESC & iPSC News 8.00 January 2, 2013
ESC & iPSC News
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TOP STORY
The RNA Pol II Elongation Factor Ell3 Marks Enhancers in ES Cells and Primes Future Gene Activation
Researchers have found that Ell3, a member of the Ell (eleven-nineteen lysine-rich leukemia gene) family of RNA Pol II elongation factors, occupies enhancers in embryonic stem (ES) cells. Ell3’s association with enhancers is required for setting up proper Pol II occupancy at the promoter-proximal regions of developmentally regulated genes and for the recruitment of the super elongation complex to these loci following differentiation signals. [Cell] Abstract | Press Release

New: Directed Differentiation of Pluripotent Stem Cells. A Cell Stem Cell Poster
PUBLICATIONS (Ranked by impact factor of the journal)

Foxa2 and H2A.Z Mediate Nucleosome Depletion during Embryonic Stem Cell Differentiation
Investigators determined the mechanisms that control global nucleosome dynamics during embryonic stem cell differentiation into endoderm. [Cell] Abstract

H2A.Z Facilitates Access of Active and Repressive Complexes to Chromatin in Embryonic Stem Cell Self-Renewal and Differentiation
Scientists showed that H2A.Z is highly enriched at promoters and enhancers and is required for both efficient self-renewal and differentiation of murine embryonic stem cells. [Cell Stem Cell] Abstract

An H3K36 Methylation-Engaging Tudor Motif of Polycomb-Like Proteins Mediates Polycomb Repressive Complex 2 Complex Targeting
In murine pluripotent stem cells, investigators identified coexistence of histone H3 lysine 36 trimethylation (H3K36me3), H3K27me3, and PHF19/polycomb-like 3 (PCL3) at a subset of poised developmental genes and demonstrated that PHF19/PCL3 Tudor function is required for optimal H3K27me3 and repression of these loci. [Mol Cell] Abstract | Press Release

Poly(ADP-Ribose) Polymerase 1 Regulates Nuclear Reprogramming and Promotes iPSC Generation without c-Myc
Poly(ADP-ribose) polymerase 1 (Parp1) catalyzes poly(ADP-ribosylation) (PARylation) and induces replication networks involved in multiple nuclear events. Using mass spectrometry and Western blotting, Parp1 and PARylation activity were intensively detected in induced pluripotent stem cells (iPSCs) and embryonic stem cells, but they were lower in mouse embryonic fibroblasts and differentiated cells. Researchers showed that knockdown of Parp1 and pharmacological inhibition of PARylation both reduced the efficiency of iPSC generation induced by Oct4/Sox2/Klf4/c-Myc. [J Exp Med] Abstract

Sleeping Beauty Transposon-Based System for Cellular Reprogramming and Targeted Gene Insertion in Induced Pluripotent Stem Cells
Researchers established induced pluripotent stem (iPS) cell reprogramming of mouse embryonic fibroblasts and human foreskin fibroblasts by transposition of OSKM (Oct4, Sox2, Klf4 and c-Myc) and OSKML (OSKM + Lin28) expression cassettes mobilized by the SB100X hyperactive transposase. The efficiency of iPS cell derivation with Sleeping Beauty (SB) transposon system was in the range of that obtained with retroviral vectors. [Nucleic Acids Res] Full Article

A PRC2 Dependent Repressive Role of PRDM14 in Human Embryonic Stem Cells and Induced Pluripotent Stem Cell Reprogramming
The authors provide evidence that PRDM14 is a direct repressor of developmental genes in human embryonic stem cells. [Stem Cells] Abstract

Human Pluripotent Stem Cell Models of Fanconi Anemia Deficiency Reveal an Important Role for Fanconi Anemia Proteins in Cellular Reprogramming and Survival of Hematopoietic Progenitors
Researchers report that Fanconi anemia (FA)-C patient specific human induced pluripotent stem cells (hiPSC) lines can be derived under normoxic conditions, albeit at much reduced efficiency. Both FANCC deficient human embryonic stem cell and hiPSC lines are capable of undergoing hematopoietic differentiation, but the hematopoietic progenitors display an increased apoptosis in culture and reduced clonogenic potential. [Stem Cells] Abstract

RARγ Is Essential for Retinoic Acid Induced Chromatin Remodeling and Transcriptional Activation in Embryonic Stem Cells
Researchers utilized retinoic acid receptor γ knockout (RARγ−/−) embryonic stem cells as a model system to analyze RARγ mediated transcriptional regulation of stem cell differentiation. [J Cell Sci] Abstract

Transient JMJD2B-Mediated Reduction of H3K9me3 Levels Improves Reprogramming of Embryonic Stem Cells into Cloned Embryos
Scientists generated mouse embryonic stem cells for the inducible expression of JMJD2B, a demethylase that primarily removes the histone 3 lysine 9 tri-methylation (H3K9me3) mark. [Mol Cell Biol] Abstract

Stemness-Related Factor Sall4 Interacts with Transcription Factors Oct-3/4 and Sox2 and Occupies Oct-Sox Elements in Mouse Embryonic Stem Cells
Researchers showed that Sall4, a member of the Spalt-like family of proteins, directly interacts with Sox2 and Oct-3/4. Sall4 in combination with Sox2 or Oct-3/4 simultaneously occupies the Oct-Sox elements in mouse embryonic stem cells. [J Biol Chem] Abstract | Full Article

New: STEMdiff™ Definitive Endoderm Kit. For Easy, Standardized hPSC Differentiation

REVIEWS
A Blueprint for Engineering Cell Fate: Current Technologies to Reprogram Cell Identity
In this review, the authors discuss current strategies to manipulate the identity of abundant and accessible cells by differentiation from an induced pluripotent state or direct conversion between differentiated states. [Cell Res] Abstract
INDUSTRY NEWS

GE Healthcare and Cellular Dynamics International Agree to Sublicense for Cellular Assay Patents
GE Healthcare Life Sciences and Cellular Dynamics International (CDI) announced that GE Healthcare has licensed CDI to develop, manufacture and sell cellular assays and models derived from induced pluripotent stem cells for use in drug discovery and toxicity screening. [Cellular Dynamics International] Press Release

Emory/Georgia Tech Regenerative Engineering and Medicine Center Awards 11 Collaborative Seed Grants
The Emory/Georgia Tech Regenerative Engineering and Medicine Center recently awarded 11 seed grants, totaling $630,000, for promising new research in regenerative medicine. The seed grants focus on how the body – including bone, muscle, nerves, blood vessels and tissues – can harness its own potential to heal or regenerate following trauma or disease. [Georgia Institute of Technology] Press Release

POLICY NEWS

US Fiscal Deal Leaves Science Vulnerable
Law-makers in Washington DC greeted the new year with a frantic deal meant to avert a fiscal crisis. But the bill that passed the Senate and the House keeps researchers on tenterhooks for at least another two months by delaying mandatory spending cuts that could threaten science funding. [US Government, United States] Press Release

South Korea’s President-Elect Promises Science Boost
South Korea’s thriving research base could be propelled to new heights if its next president makes good on her election promises. Sixty-year-old Park, who studied electronic engineering at Sogang University in Seoul, pledged during her campaign that science and technology policy would become the cornerstone of her government’s work. [South Korean President, South Korea] Press Release

EVENTS
NEW Keystone Symposia on Molecular and Cellular Biology: Stem Cell Regulation in Homeostasis and Disease
February 24-March 1, 2013
Banff, Canada

Visit
our events page to see a complete list of events in the ESC & iPSC community.
JOB OPPORTUNITIES

Scientist or Engineer – hPSC Bioengineer (STEMCELL Technologies, Inc.)

MSc – Future Opportunities (STEMCELL Technologies, Inc.)

PhD – Future Opportunities (STEMCELL Technologies, Inc.)

Postdoctoral Position – Tumor Stem Cells (Cancer Institute of New Jersey)

Postdoctoral Position – Stem Cells and Epigenetics (Mount Sinai School of Medicine)

Senior QC Analyst/Assay Development Associate (StemCells, Inc.)

Postdoctoral Research Fellowship – Stem Cell Biology (National Institute of Environmental Health Sciences, National Institutes of Health)

Postdoctoral Position – Cardiovascular Regenerative Therapies (University of California, Los Angeles)

Senior Postdoctoral Position (Vrije Universiteit Brussel, Department of Biology)


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