Volume 8.49 | Dec 11

ESC & iPSC News 8.49 December 11, 2013
ESC & iPSC News
     In this issue: Publications | Reviews | Industry News | Policy News | Events | Jobs
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TOP STORY
Induction of a Human Pluripotent State with Distinct Regulatory Circuitry that Resembles Preimplantation Epiblast
Investigators used a combination of three small-molecule inhibitors to sustain human embryonic stem cells (hESCs) in a LIF signaling-dependent hESC state with elevated expression of NANOG and epiblast-enriched genes such as KLF4, DPPA3, and TBX3. [Cell Stem Cell] Abstract | Graphical Abstract | Press Release
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PUBLICATIONS (Ranked by impact factor of the journal)
Human iPSC-Based Modeling of Late-Onset Disease via Progerin-Induced Aging
Scientists describe a strategy for inducing aging-related features in human induced pluripotent stem cell (iPSC)-derived lineages and apply it to the modeling of Parkinson’s disease. [Cell Stem Cell]
Abstract | Graphical Abstract | Press Release

ELABELA: A Hormone Essential for Heart Development Signals via the Apelin Receptor
Researchers report the discovery and characterization of a gene, ELABELA (ELA), encoding a conserved hormone of 32 amino acids. Present in human embryonic stem cells, ELA is expressed at the onset of zebrafish zygotic transcription and is ubiquitous in the naive ectodermal cells of the embryo. [Dev Cell] Abstract | Graphical Abstract | Press Release

Heart-Specific Stiffening in Early Embryos Parallels Matrix and Myosin Expression to Optimize Beating
Researchers focused on the spontaneously beating embryonic heart and sparsely cultured cardiomyocytes, including cells derived from pluripotent stem cells. Tissue elasticity, Et, increases daily for heart to 1-2 kPa by embryonic day 4 (E4), and although this is ~10-fold softer than adult heart, the beating contractions of E4 cardiomyocytes prove optimal at ~Et,E4 both in vivo and in vitro. [Curr Biol] Abstract | Graphical Abstract

Dual Integrase Cassette Exchange, an Efficient System for Iterative Genomic Editing in Human Pluripotent Stem Cells
The authors describe a system for precise genetic modification of human embryonic stem cells and induced pluripotent stem cells. They identified a novel human locus, H11, located in a safe, intergenic, transcriptionally active region of chromosome 22, as the recipient site, to provide robust, ubiquitous expression of inserted genes. [Nucleic Acid Res] Abstract

Novel Insights into Embryonic Stem Cell Self-Renewal Revealed through Comparative Human and Mouse Systems Biology Networks
Investigators explored the molecular basis of human embryonic stem cells self-renewal using Bayesian network machine learning to integrate cell-type-specific, high-throughput data for gene function discovery. [Stem Cells] Abstract

STAT3 Phosphorylation at Tyrosine 705 and Serine 727 Differentially Regulates Mouse ES Cell Fates
Investigators examined the roles of STAT3 phosphorylated Tyrosine 705 and Serine 727 residues in regulating mouse embryonic stem (mES) cell identities, using a small molecule-based system to post-translationally modulate the quantity of transgenic STAT3 in STAT3-/- mES cells. [Stem Cells] Abstract

Patient-Specific Naturally Gene-Reverted Induced Pluripotent Stem Cells in Recessive Dystrophic Epidermolysis Bullosa
Scientists showed that revertant recessive dystrophic epidermolysis bullosa (RDEB) keratinocytes expressing functional type VII collagen can be reprogrammed into induced pluripotent stem cells (iPSCs) and that self-corrected RDEB iPSCs can be induced to differentiate into either epidermal or hematopoietic cell populations. [J Invest Dermatol] Abstract

Epigenetic Regulation of miR-302 by JMJD1C Inhibits Neural Differentiation of Human Embryonic Stem Cells
It has been recently reported that the regulatory circuitry formed by OCT4, mircoRNA-302 (miR-302), and NR2F2 controls both pluripotency and neural differentiation of human embryonic stem cells (hESCs). Scientists showed that JMJD1C, a H3K9 demethylase expressed in hESCs, directly interacts with this circuitry. [J Biol Chem] Abstract | Full Article

APP Processing in Human Pluripotent Stem Cell-Derived Neurons Is Resistant to NSAID-Based γ-Secretase Modulation
Reasoning that the nonhuman and nonneuronal cells typically used in pharmaceutical compound validation might not adequately reflect the drug responses of human neurons, investigators used human pluripotent stem cell-derived neurons from Alzheimer’s disease patients and unaffected donors to explore the efficacy of nonsteroidal anti-inflammatory drug (NSAID)-based γ-secretase modulation. [Stem Cell Rep]
Full Article | Graphical Abstract | Press Release

Derivation and FACS-Mediated Purification of PAX3+/PAX7+ Skeletal Muscle Precursors from Human Pluripotent Stem Cells
The authors describe a method based on the use of a small-molecule GSK3β inhibitor to derive skeletal muscle from several human pluripotent stem cell (hPSC) lines. They showed that early GSK3β inhibition is sufficient to create the conditions necessary for highly effective derivation of muscle cells. [Stem Cell Rep] Full Article | Graphical Abstract | Press Release

Take the "if" out of "diff": Reduce variability when differentiating to endothelial cells
 
REVIEWS
Exploiting Pluripotent Stem Cell Technology for Drug Discovery, Screening, Safety, and Toxicology Assessments
This review discusses some of the benefits of using pluripotent stem cells for drug discovery and safety studies as well as some of the recent applications of stem cells in drug screening studies. [Adv Drug Deliv Rev] Abstract

Defining Synthetic Surfaces for Human Pluripotent Stem Cell Culture
This review summarizes the recent reports of defined surfaces for human pluripotent stem cell culture and focuses on the cell adhesion molecules and extracellular matrix proteins involved. [Cell Regeneration]
Full Article

Visit our reviews page to see a complete list of reviews in the ESC & iPSC research field.

Hear from the leaders in the Cell Culture sector!
 
INDUSTRY NEWS
The Hamner Institutes and Cellular Dynamics Collaborate to Develop In Vitro Assays Using Human iPS Cell-Derived Hepatocytes
The Hamner Institutes for Health Sciences announced a collaborative agreement with Cellular Dynamics International (CDI) to develop predictive in vitro screening assays for chemical, environmental and pharmaceutical toxicology assessments that utilize CDI’s human induced pluripotent stem (iPS) cell-derived hepatocytes. [Cellular Dynamics International, Inc.] Press Release

NASA, CASIS Make Space Station Accessible for Stem Cell Research
NASA and the Center for the Advancement of Science in Space (CASIS) are enabling research aboard the International Space Station that could lead to new stem cell-based therapies for medical conditions faced on Earth and in space. [National Aeronautics and Space Administration] Press Release

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POLICY NEWS
Overhaul Recommended for Gene-Therapy Review
The US National Institutes of Health (NIH) should refocus its oversight of gene-transfer research, the US Institute of Medicine says. The analysis concludes that the NIH’s Recombinant DNA Advisory Committee, or RAC, should no longer review most gene-therapy research. [Nature News] Editorial

Italian Court Rules Science Advisers Unlawful
The regional administrative court of Lazio, Italy, ruled unlawful the scientific committee that had advised the health minister not to proceed with a planned €3 million clinical trial of a controversial stem-cell therapy. [Nature News] Editorial

Stem Cell Research Faces Budget Crunch
The cash-strapped federal government’s ability to fund stem cell research has become severely limited, the top official in charge of the research told attendees at the World Stem Cell Summit. So the private sector is being encouraged to play a larger role, to take over some functions, provide order and reduce needless duplication of effort. [San Diego Union-Tribune] Editorial
 
EVENTS
NEW Gordon Research Seminar: Craniofacial Morphogenesis & Tissue Regeneration
March 29-30, 2014
Lucca, Italy

Visit our events page to see a complete list of events in the ESC & iPSC community.
 
JOB OPPORTUNITIES
NEW Postdoctoral Associate – Stem Cell Biology (State University of New York)

Postdoctoral Fellow – Cardiovascular Tissue Engineering (Stanford University School of Medicine)

Clinical MD – Clinical Development Program for Oncology and Autoimmune Diseases (Immunomedics, Inc.)

Chief Medical Officer – Novel Therapeutics in Oncology and Autoimmune Disease (Immunomedics, Inc.)

Postdoctoral Position – RNA and Stem Cell Biology (Cancer Research Centre of Lyon)

Research Associate – Stem Cells & Regenerative Medicine (King’s College London)

Postdoctoral Fellow – Liver Tissue Engineering (Harvard Medical School)

Postdoctoral Position – Stem Cells and Epigenetics (Icahn School of Medicine at Mount Sinai)

Assistant Director (Wake Forest Institute for Regenerative Medicine)

Postdoctoral Position – Human Stem Cells (Sabine Costagliola-ULB)

Scientist – Particle Chemistry (STEMCELL Technologies Inc.)

Research Technologist – Human Pluripotent Stem Cell Products (STEMCELL Technologies Inc.)

Research Associate/Scientist – Antibodies Group (STEMCELL Technologies Inc.)


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