Volume 9.01 | Jan 15

ESC & iPSC News 9.01 January 15, 2014
ESC & iPSC News
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Cell-Autonomous Correction of Ring Chromosomes in Human Induced Pluripotent Stem Cells
Researchers generated human induced pluripotent stem cells from patient fibroblasts containing ring chromosomes with large deletions and found that reprogrammed cells lost the abnormal chromosome and duplicated the wild-type homologue through the compensatory uniparental disomy mechanism. [Nature]
Abstract | Press Release
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PUBLICATIONS (Ranked by impact factor of the journal)
Genetic Exploration of the Exit from Self-Renewal Using Haploid Embryonic Stem Cells
Self-renewal circuitry in embryonic stem cells (ESCs) is increasingly defined. How the robust pluripotency program is dissolved to enable fate transition is less appreciated. The authors developed a forward genetic approach using haploid ESCs. They created libraries of transposon integrations and screened for persistent self-renewal in differentiation-permissive culture. [Cell Stem Cell] Abstract | Graphical Abstract | Press Release

Efficient Endoderm Induction from Human Pluripotent Stem Cells by Logically Directing Signals Controlling Lineage Bifurcations
Researchers revisited signals driving induction and anterior-posterior patterning of definitive endoderm to generate a coherent roadmap for endoderm differentiation. With striking temporal dynamics, BMP and Wnt initially specified anterior primitive streak (progenitor to endoderm), yet, 24 hours later, suppressed endoderm and induced mesoderm. [Cell Stem Cell] Abstract | Graphical Abstract

Snail1-Dependent Control of Embryonic Stem Cell Pluripotency and Lineage Commitment
Scientists demonstrated using isogenic pairs of conditional knockout mouse embryonic stem cells, that Snail1 exerts Wnt- and epithelial-mesenchymal transition independent control over the stem cell transcriptome without affecting self-renewal or pluripotency-associated functions. [Nat Commun] Abstract

Mutant Astrocytes Differentiated from Rett Syndrome Patients-Specific iPSCs Have Adverse Effects on Wild Type Neurons
Scientists showed that Rett syndrome (RTT) induced pluripotent stem cell (iPSC) lines can be efficiently differentiated into astroglial progenitors and glial fibrillary acidic protein expressing astrocytes that maintain isogenic status, that mutant RTT astrocytes carrying three different RTT mutations and their conditioned media have adverse effects on the morphology and function of wild type neurons, and that the glial effect on neuronal morphology is independent of the intrinsic neuronal deficit in mutant neurons. [Hum Mol Genet] Abstract

Characterization and Molecular Profiling of PSEN1 Familial Alzheimer’s Disease iPSC-Derived Neural Progenitors
In order to elucidate pathways downstream of Presenilin 1 (PSEN1), the authors characterized neural progenitor cells derived from familial Alzheimer’s disease mutant PSEN1 subjects. They generated induced pluripotent stem cells from affected and unaffected individuals from two families carrying PSEN1 mutations. [PLoS One]
Full Article | Press Release

Spatially Defined InsP3-Mediated Signaling in Embryonic Stem Cell-Derived Cardiomyocytes
The authors aimed to determine how 1,4,5-trisphosphate (InsP3) receptors can translate Ca2+ release into a depolarization of the plasma membrane and consequently arrhythmic activity. They used embryonic stem cell-derived cardiomyocytes as a model system since their spontaneous electrical activity depends on InsP3-mediated Ca2+ release. [PLoS One] Full Article

Kinetic Analysis of Deviation from the Undifferentiated State in Colonies of Human Induced Pluripotent Stem Cells on Feeder Layers
Scientists focused on deviation from the undifferentiated state in human induced pluripotent stem cells (hiPSC) colonies during culture with feeder cells. Deviated cells from the undifferentiated state of hiPSCs in cultures with SNL and MEF feeder cells were observed at the center and periphery of the colonies, respectively, accompanied by dramatic changes in the cell morphology from small to large flattened shapes. [Biotechnol Bioeng] Abstract

Long-Term Electrophysiological Activity and Pharmacological Response of a Human Induced Pluripotent Stem Cell-Derived Neuron and Astrocyte Co-Culture
Researchers used a multi-electrode array system to investigate the effects of the co-culture of rat astrocytes with human induced pluripotent stem cell-derived neurons on the long-term culture, spontaneous firing activity, and drug responsiveness effects. [Biochem Biophys Res Commun] Abstract

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Considerations for Pre-Clinical Models and Clinical Trials of Pluripotent Stem Cell-Derived Cardiomyocytes
The authors review the main questions that should be considered before translating pluripotent stem cell (PSC)-derived cardiomyocytes into clinical investigations, including the development of good manufacturing practice-level PSC lines, the development of efficient protocols to generate pure populations of cardiac myocytes, and the development of techniques to improve the retention and survival rate of transplanted cells. [Stem Cell Res Ther] Abstract

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Athersys Announces New Patents in Japan for Stem Cell and Regenerative Medicine Technology
Athersys, Inc. announced that it has been granted patents from the Japan Patent Office for several inventions involving its proprietary cell therapy technology. [Athersys, Inc.] Press Release

The International Society for Stem Cell Research (ISSCR) Announces the 2014 Recipients of the McEwen Award for Innovation, the ISSCR-BD Biosciences Outstanding Young Investigator and the ISSCR Public Service Awards
The McEwen Award for Innovation, supported by the McEwen Center for Regenerative Medicine, recognizes original thinking and groundbreaking research pertaining to stem cells or regenerative medicine that opens new avenues of exploration toward the understanding or treatment of human disease or affliction. The ISSCR-BD Biosciences Outstanding Young Investigator Award recognizes exceptional achievements by an ISSCR member and investigator in the early part of their independent career in stem cell research. The ISSCR Public Service Award is given in recognition of outstanding contributions of public service to the fields of stem cell research and regenerative medicine. [The International Society for Stem Cell Research] Press Release

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Leaked Files Slam Stem-Cell Therapy
A series of damning documents seen by Nature expose deep concerns over the safety and efficacy of the controversial stem-cell therapy promoted by Italy’s Stamina Foundation. The leaked papers reveal the true nature of the processes involved, long withheld by Stamina’s president, Davide Vannoni. Other disclosures show that the successes claimed by Stamina for its treatments have been over-stated. [Nature News] Editorial

U.K. Researchers Ask the Public to Share Medical Data
U.K. medical research charities urged residents to allow scientists to access patient data from the country’s National Health Service. Making their plea through colorful advertisements in the national press, the charities weighed in on a raging national debate. [ScienceInsider] Editorial
NEW Cell Line Development Asia 2014
May 20-23, 2014
Shanghai, China

Visit our events page to see a complete list of events in the ESC & iPSC community.
NEW Postdoctoral Position – Epigenetic Regulation of Heart Formation Using Human ES & iPS Cells (University of Pittsburgh)

NEW Postdoctoral Associate – Hematopoietic Differentiation (Northwestern University Feinberg School of Medicine)

NEW Postdoctoral Researcher – Stem Cell Biology (University of Luxembourg)

Postdoctoral Associate – Stem Cell Biology (State University of New York)

Postdoctoral Fellow – Cardiovascular Tissue Engineering (Stanford University School of Medicine)

Clinical MD – Clinical Development Program for Oncology and Autoimmune Diseases (Immunomedics, Inc.)

Chief Medical Officer – Novel Therapeutics in Oncology and Autoimmune Disease (Immunomedics, Inc.)

Postdoctoral Fellow – Liver Tissue Engineering (Harvard Medical School)

Research Technologist – Human Pluripotent Stem Cell Products (STEMCELL Technologies Inc.)

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