Volume 9.04 | Feb 5

ESC & iPSC News 9.04 February 5, 2014
ESC & iPSC News
     In this issue: Publications | Reviews | Industry News | Policy News | Events | Jobs
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TOP STORY
Cell Cycle Speed Is Key to Making Aging Cells Young Again
Researchers identified a major obstacle to converting cells back to their youthful state – the speed of the cell cycle, or the time required for a cell to divide. When the cell cycle accelerates to a certain speed, the barriers that keep a cell’s fate on one path diminish. [Press release from the Yale School of Medicine discussing online publication in Cell] Press Release | Video | Abstract | Graphical Abstract
Webinar: Groundbreaking Applications for Feeder-Free hESC & hiPSC Culture. Watch Now.
 
PUBLICATIONS (Ranked by impact factor of the journal)
Coordinated Repressive Chromatin-Remodeling of Oct4 and Nanog Genes in RA-Induced Differentiation of Embryonic Stem Cells Involves Receptor-Interacting Protein 140
Investigators examined retinoic acid (RA) repression of the Nanog gene and compares the results with RA repression of the Oct4 gene on the chromatin level. The results show a loose nucleosome array on the Nanog gene promoter in undifferentiated embryonic stem cells. [Nucleic Acid Res] Abstract

The Influence of a Spatiotemporal 3D Environment on Endothelial Cell Differentiation of Human Induced Pluripotent Stem Cells
Current endothelial cell (EC) differentiation protocols are inefficient, and the phenotypes of the differentiated ECs are only briefly stable, which significantly inhibits their utility for basic science research. A remarkably more efficient human induced pluripotent stem cells-EC differentiation protocol that incorporates a three-dimensional fibrin scaffold is presented. [Biomaterials] Abstract

Cleavage of E-Cadherin and β-Catenin by Calpain Affects Wnt Signaling and Spheroid Formation in Suspension Cultures of Human Pluripotent Stem Cells
Investigators assessed gene expression of human embryonic stem cells and induced pluripotent stem cells grown in surface-adherent culture versus free-floating suspension culture spheroids. [Mol Cell Proteomics] Abstract

Regulation of iPS Cell Induction by Wnt/β-Catenin Signaling
Researchers report that Wnt/β-catenin enhances induced pluripotent stem (iPS) cells induction at the early stage of reprogramming. The augmented reprogramming induced by β-catenin is not due to increased total cell population or activation of c-Myc. [J Biol Chem] Abstract

Generation of Disease-Specific Induced Pluripotent Stem Cells from Patients with Rheumatoid Arthritis and Osteoarthritis
A 4-in-1 lentiviral vector containing Oct4, Sox2, Klf4, and c-Myc was transduced into rheumatoid arthritis (RA) and osteoarthritis (OA) fibroblast-like synoviocytes (FLSs) isolated from the synovia of two RA patients and two OA patients. Immunohistochemical staining and real-time PCR studies were performed to demonstrate the pluripotency of the induced pluripotent stem cells. [Arthritis Res Ther] Abstract | Full Article

Generation of Human Induced Pluripotent Stem (iPS) Cells in Serum- and Feeder-Free Defined Culture and TGF-Β1 Regulation of Pluripotency
Scientists successfully generated human iPS cells from human dental pulp cells using Yamanaka’s factors (Oct3/4, Sox2, Klf4, and c-Myc) with retroviral vectors in serum- and feeder-free defined culture conditions. [PLoS One] Full Article

Reprogramming of COPD Lung Fibroblasts through Formation of Induced Pluripotent Stem Cells (iPSCs)
Reprogramming somatic cells to iPSCs eliminates many epigenetic modifications that characterize differentiated cells. Investigators tested whether functional differences between COPD and non-COPD fibroblasts could be reduced utilizing this approach. [Am J Physiol Lung Cell Mol Physiol] Abstract

Adipocytes Derived from PA6 Cells Reliably Promote the Differentiation of Dopaminergic Neurons from Human Embryonic Stem Cells
Researchers report that the coculture of human embryonic stem cells with PA6-derived adipocytes reliably induces their differentiation into midbrain dopaminergic neurons. [J Neurosci Res] Abstract

Chemically Induced Specification of Retinal Ganglion Cells from Human Embryonic and Induced Pluripotent Stem Cells
A novel, stepwise chemical protocol is described for the differentiation of human embryonic stem cells and induced pluripotent stem cells into functional retinal ganglion cells. [Stem Cell Transl Med] Abstract

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Take the "if" out of "diff": Reduce variability when differentiating to endothelial cells
 
REVIEWS
Development-Inspired Reprogramming of the Mammalian Central Nervous System
Although reprogramming with known transcription factors is a comparatively blunt tool, researchers have used knowledge of normal neuronal development to identify suites of factors that can convert mouse or human non-neuronal cells into induced neuronal cells showing class-specific features. [Science] Abstract

Role of p75 Neurotrophin Receptor in Stem Cell Biology: More than Just a Marker
The authors give an overview of the current knowledge on p75NTR in stem cells, ranging from embryonic to adult stem cells, and cancer stem cells. In an attempt to address its potential role in the control of stem cell biology, the molecular mechanisms underlying p75NTR signaling in different models are also highlighted. [Cell Mol Life Sci] Abstract

Great Expectations: Autism Spectrum Disorder and Induced Pluripotent Stem Cell Technologies
New applications of induced pluripotent stem cell technology to research on complex idiopathic conditions raise several important ethical and social considerations for potential research participants and their families. Investigators examine these issues through the lens of emerging research on autism spectrum disorder. [Stem Cell Rev] Abstract

Visit our reviews page to see a complete list of reviews in the ESC & iPSC research field.
 
INDUSTRY NEWS
EBiSC – The First European Bank for Induced Pluripotent Stem Cells
Pharmaceutical companies who are members of the European Federation of Pharmaceutical Industries and Associations have joined forces with small and medium-sized enterprises and academia in an Innovative Medicines Initiative supported public private partnership project to establish the leading facility for the storage and distribution of induced pluripotent stem cells in Europe. [European Bank for Induced Pluripotent Stem Cells (EBiSC)] Press Release

Stem Cell Agency Funds More than $66 Million in Research Including New Genomics Initiative
A team bringing together experts and investigators from seven different major California institutions has been awarded $40 million to create a new Center of Excellence in Stem Cell Genomics, by California’s stem cell agency, the California Institute for Regenerative Medicine. [California Institute for Regenerative Medicine]
Press Release

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POLICY NEWS
Row Over Controversial Stem-Cell Procedure Flares Up Again
Top scientists in Italy have called on the health minister Beatrice Lorenzin to reconsider the composition of the new scientific advisory committee she has proposed to assess a controversial stem-cell therapy offered by the Stamina Foundation. [Nature News] Editorial

Hollande Pledges to Avoid Cuts to France’s Science Funding
French President François Hollande promised to spare the research and higher education budget from savings of €50 billion that his government has pledged to find over the next three years to reign in its massive public deficit. [Nature News] Editorial

Obama Promises Action and Seeks a Science-Funding Boost
US President Barack Obama says 2014 will be a “year of action” in which he plans to use his executive authority to enact new policies, while seeking greater cooperation from the sharply divided Congress. [Nature News] Editorial

Elsevier Opens Its Papers to Text-Mining
Academics: prepare your computers for text-mining. Publishing giant Elsevier says that it has now made it easy for scientists to extract facts and data computationally from its more than 11 million online research papers. Other publishers are likely to follow suit this year, lowering barriers to the computer-based research technique. [Nature News] Editorial
 
EVENTS
NEW Clinical Translation of Stem Cells 2014
April 21-22, 2014
Palm Desert, California

Visit our events page to see a complete list of events in the ESC & iPSC community.
 
JOB OPPORTUNITIES
NEW Postdoctoral Scholar – Cell-Cell Interactions in Mammalian Development (University of California, San Francisco)

NEW Postdoctoral Fellow – Human Induced Pluripotent Stem Cell Initiative (European Bioinformatics Institute)

NEW Lab Technologist – Human Pluripotent Stem Cells (STEMCELL Technologies Inc.)

Senior Research Scientist – Immunogenicity and Epigenetic Regulation of hiPSC-Derived Endothelial Cells (Magdi Yacoub Institute)

Postdoctoral Position – Stem Cell Signaling and Epigenetics (Fondazione Istituto Nazionale Genetica Molecolare)

Postdoctoral Position – Systems Biology of Cellular Reprogramming (University of California – San Diego)

Postdoctoral Position – Stem Cells and Epigenetics (Mount-Sinai)

Postdoctoral Fellow – Morphogenetic Signaling Pathways in Development and Disease (University of Rochester Medical Center)

Postdoctoral Position – Epigenetic Regulation of Heart Formation Using Human ES & iPS Cells (University of Pittsburgh)


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