Volume 9.16 | Apr 30

ESC & iPSC News 9.16 April 30, 2014
ESC & iPSC News
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First Disease-Specific Human Embryonic Stem Cell Line by Nuclear Transfer
A team of scientists has created the first disease-specific embryonic stem cell line with two sets of chromosomes. The scientists derived embryonic stem cells by adding the nuclei of adult skin cells to unfertilized donor oocytes using a process called somatic cell nuclear transfer. Embryonic stem cells were created from one adult donor with type 1 diabetes and a healthy control. [Press release from the New York Stem Cell Foundation (NYSCF) discussing online prepublication in Nature] Press Release | Abstract
Enter to Win a Travel Award to ISSCR 2014
PUBLICATIONS (Ranked by impact factor of the journal)
Human Embryonic-Stem-Cell-Derived Cardiomyocytes Regenerate Non-Human Primate Hearts
Scientists showed that human embryonic-stem-cell-derived cardiomyocytes (hESC-CMs) can be produced at a clinical scale (more than one billion cells per batch) and cryopreserved with good viability. Using a non-human primate model of myocardial ischemia followed by reperfusion, they showed that that cryopreservation and intra-myocardial delivery of one billion hESC-CMs generates extensive remuscularization of the infarcted heart. [Nature] Abstract | Press Release

Deep Transcriptome Profiling of Mammalian Stem Cells Supports a Regulatory Role for Retrotransposons in Pluripotency Maintenance
The authors performed deep profiling of the nuclear and cytoplasmic transcriptomes of human and mouse stem cells, identifying a class of previously undetected stem cell-specific transcripts. They showed that long terminal repeat-derived transcripts contribute extensively to the complexity of the stem cell nuclear transcriptome. [Nat Genet] Abstract | Press Release

Reprogramming Committed Murine Blood Cells to Induced Hematopoietic Stem Cells with Defined Factors
Scientists showed that transient expression of six transcription factors Run1t1, Hlf, Lmo2, Prdm5, Pbx1, and Zfp37 imparts multilineage transplantation potential onto otherwise committed lymphoid and myeloid progenitors and myeloid effector cells. Inclusion of Mycn and Meis1 and use of polycistronic viruses increase reprogramming efficacy. [Cell] Abstract | Graphical Abstract | Press Release

Klf4 and Klf5 Differentially Inhibit Mesoderm and Endoderm Differentiation in Embryonic Stem Cells
Using microarrays and chromatin immunoprecipitation coupled to ultra-high-throughput DNA sequencing, researchers showed that Krüppel-like factor (Klf)4 negatively regulates the expression of endodermal markers in the undifferentiated embryonic stem cells, including transcription factors involved in the commitment of pluripotent stem cells to endoderm differentiation. [Nat Commun] Abstract

3D In Vitro Model of a Functional Epidermal Permeability Barrier from Human Embryonic Stem Cells and Induced Pluripotent Stem Cells
Scientists demonstrated the generation of human epidermal equivalents (HEEs) from human embryonic stem cells and induced pluripotent stem cells. HEEs are structurally similar to native epidermis, with a functional permeability barrier. [Stem Cell Rep] Full Article | Press Release

Primordial Dwarfism Gene Maintains Lin28 Expression to Safeguard Embryonic Stem Cells from Premature Differentiation
The authors showed that La ribonucleoprotein domain family, member 7 (Larp7) deficiency likely does not significantly increase positive transcription elongation factor b activity. They further discovered that Larp7 knockdown does not affect pluripotency but instead primes embryonic stem cells for differentiation via downregulation of Lin28, a positive regulator of organismal growth. [Cell Rep] Full Article | Graphical Abstract

Epigenome Rejuvenation: HP1β Mobility as a Measure of Pluripotent and Senescent Chromatin Ground States
The authors measured the dynamics of an essential epigenetic modifier, HP1β, in human cells at different stages of differentiation using fluorescence recovery after photobleaching. They found that HP1β mobility is similar in human embryonic stem cells and iPS cells where it is more mobile compared to fibroblasts; HP1β is less mobile in senescent fibroblasts than in young (dividing) fibroblasts. [Sci Rep] Full Article

Differentiation of Mouse Induced Pluripotent Stem Cells into Alveolar Epithelial Cells In Vitro for Use In Vivo
Researchers modified a two-step protocol for embryonic stem cells that resulted in a yield of 9% surfactant protein C+ alveolar epithelial type II cells from mouse induced pluripotent stem cells in a 12-day period. [Stem Cells Transl Med] Abstract

Increased Genomic Integrity of an Improved Protein-Based Mouse Induced Pluripotent Stem Cell Method Compared with Current Viral-Induced Strategies
Investigators established an improved method for protein reprogramming by using partially purified reprogramming proteins, resulting in more efficient generation of induced pluripotent stem cells from C57/BL6J mouse hepatocytes than using protein extracts. [Stem Cells Transl Med] Abstract

Webinar: Modeling Human Neurological Disease with iPS Cells
Wnt of the Two Horizons: Putting Stem Cell Self-Renewal and Cell Fate Determination into Context
The author assesses the seemingly contradictory findings regarding Wnt signaling in either self-renewal or differentiation in embryonic stem cells and then explicate cellular scenarios that determine the context by which Wnt ligands exert their differential effects. [Stem Cells Dev] Abstract

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Athersys Announces Results from Phase II Study of MultiStem® Cell Therapy for Ulcerative Colitis
Athersys, Inc. announced interim results from the Phase II clinical study of the administration of Athersys’ MultiStem® cell therapy to treatment refractory ulcerative colitis (UC) patients being conducted by Pfizer, Inc. The cell therapy failed to show meaningful benefit, following a single administration, in patients suffering from chronic, moderate-to-severe UC who have failed other therapies. [Athersys, Inc.] Press Release

Advanced Cell Technology’s Chief Scientific Officer Dr. Robert Lanza Named One of TIME Magazine’s 100 Most Influential People in the World
Advanced Cell Technology, Inc. announced that the company’s Chief Scientific Officer, Robert Lanza, MD, has been selected for the 2014 TIME 100 annual list of the hundred most influential people in the world. [Advanced Cell Technology, Inc.] Press Release

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Investigator of Controversial Stem-Cell Study Resigns
The head of a Japanese committee investigating claims that stem cells could be made using mechanical stress or acid resigned from the committee today over anonymous allegations that at least one of his own papers contained problematic data. He says he resigned out of concern that the incident could complicate the current investigation. [Nature News] Editorial

NSF’s Science Board Criticizes Bill to Alter Agency’s Programs
The presidentially appointed oversight body to the National Science Foundation (NSF) has taken the unprecedented step of publicly criticizing pending legislation that it feels would be harmful to the $7 billion research agency. [ScienceInsider] Editorial
NEW The 2014 Regenerative Medicine Event: Stem Cell Reprogramming
June 2, 2014
London, United Kingdom

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NEW Postdoctoral Position – Functional Characterization of Neurons Derived from Human Pluripotent Stem Cells (Radboud University Nijmegen)

NEW Postdoctoral Associate – Hematopoietic Differentiation (Northwestern University Feinberg School of Medicine)

NEW Research Technologist – hPSC (STEMCELL Technologies Inc.)

Postdoctoral Position – Basic and Translational Neuroscience/Stem Cell Research (University of California, Davis)

Full Professor – Stem Cell Research (HUST Tongji Medical College)

Postdoctoral Position – Stem Cell Biology (Yale Stem Cell Center)

Postdoctoral Research Fellow / Research Officer – Stem Cell Research (Bioprocessing Technology Institute)

Postdoctoral Position – TGFβ-Signaling Pathway in Embryonic Stem Cells (Tsinghua University)

Stem Cell Research Technician (Van Andel Research Institute)

Director of GMP/GLP Quality Operations (University of Pennsylvania, Perelman School of Medicine)

Postdoctoral Position – Human Stem Cell Biology and Hematopoiesis (Johns Hopkins University School of Medicine)

Research Associate – Cell Separation (STEMCELL Technologies Inc.)

Research Technologist – Pluripotent Stem Cells (STEMCELL Technologies Inc.)

Research Technologist – PSC Biology and Bioengineering (STEMCELL Technologies Inc.)

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