Volume 9.39 | Oct 8

ESC & iPSC News 9.39 October 8, 2014
ESC & iPSC News
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Dax1 and Nanog Act in Parallel to Stabilize Mouse Embryonic Stem Cells and Induced Pluripotency
Researchers report that Dax1 acts in parallel with Nanog to regulate mouse embryonic stem cell (mESC) identity. Dax1 stable knockdown mESCs are predisposed towards differentiation but do not lose pluripotency, whereas Dax1 overexpression supports leukemia inhibitory factor-independent self-renewal. [Nat Commun]
Full Article
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PUBLICATIONS (Ranked by impact factor of the journal)
Mouse Embryonic Stem Cell-Derived Cells Reveal Niches that Support Neuronal Differentiation in the Adult Rat Brain
Embryoid body (EB) cells derived from embryonic stem cells are endowed with a high potential to respond to specification and neuralization signals of the embryo. To identify microenvironments in the postnatal and adult rat brain with the capacity to support neuronal differentiation, researchers transplanted dissociated EB cells to conventional neurogenic and non-neurogenic regions. [Stem Cells] Abstract

An AAVS1-Targeted Minigene Platform for Correction of iPSCs from All Five Types of Chronic Granulomatous Disease
Investigators generated induced pluripotent stem cells (iPSCs) from peripheral blood CD34+ hematopoietic stem cells of patients with each of five chronic granulomatous disease genotypes. [Mol Ther] Abstract

Small Molecule-Directed Specification of Sclerotome-Like Chondroprogenitors and Induction of a Somitic Chondrogenesis Program from Embryonic Stem Cells
Pluripotent embryonic stem cells (ESCs) generate rostral paraxial mesoderm-like progeny in five to six days of differentiation induced by Wnt3a and Noggin (Nog). Researchers report that canonical Wnt signaling introduced either by forced expression of activated β-catenin, or the small-molecule inhibitor of Gsk3, CHIR99021, satisfied the need for Wnt3a signaling, and that the small-molecule inhibitor of BMP type I receptors, LDN193189, was able to replace Nog. [Development] Abstract | Press Release

Sonic Hedgehog Increases the Skin Wound Healing Ability of Mouse Embryonic Stem Cells through Micro RNA 200 Family
Although shh is important in maintaining the stem cell, the recovery effect of mESCs with shh has not yet been elucidated. Scientists investigated the effect of mESCs with shh in skin recovery in vivo as well as the related intracellular signal pathways in vitro. [Br J Pharmacol] Abstract

Cartilage Repair Using Human Embryonic Stem Cell-Derived Chondroprogenitors
Scientists developed a 14-day culture protocol under potential GMP, chemically defined conditions to generate chondroprogenitors from human embryonic stem cells. [Stem Cells Transl Med] Abstract

Engineering Efficient Retinal Pigment Epithelium Differentiation from Human Pluripotent Stem Cells
The authors sought to identify, quantify, and reduce the sources of variability in human embryonic stem cell-retinal pigment epithelium (RPE) differentiation. By monitoring the emergence of pigmented cells over time, they show how the cell line, passaging method, passage number, and seeding density have a significant and reproducible effect on the RPE yield. [Stem Cells Transl Med] Abstract

Deep Sequencing Reveals Low Incidence of Endogenous LINE-1 Retrotransposition in Human Induced Pluripotent Stem Cells
Scientists verified long interspersed element-1 (LINE-1) expression during and after reprogramming as well as potential somatic insertions driven by the most active human endogenous LINE-1 subfamily. [PLoS One]
Full Article

Investigation of Human Embryonic Stem Cell-Derived Keratinocytes as an In Vitro Research Model for Mechanical Stress Dynamic Response
Researchers investigated the use of keratinocytes derived from human embryonic stem cells as a reliable alternative model to HaCaT for study of the effects of mechanical tension. [Stem Cell Rev] Abstract

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Epigenetic Memory in Somatic Cell Nuclear Transfer and Induced Pluripotency: Evidence and Implications
The authors discuss recent work on epigenetic memory retained in pluripotent cells derived by somatic cell nuclear transfer and transcription factor mediated reprogramming, and the challenges attached to it. [Differentiation] Abstract

Visit our reviews page to see a complete list of reviews in the ESC & iPSC research field.
NIBSC Signs Agreement with UCL
A Memorandum of Understanding has been signed between the National Institute for Biological Standards and Control (NIBSC) and University College London (UCL). The memorandum recognizes the tremendous promise of gene therapy, stem cell therapy and tissue engineering in preventing and treating illness, restoring health and saving lives. [National Institute for Biological Standards and Control] Press Release

Midwestern Regional Medical Center Recognized for Exemplary Stem Cell Treatment and Cell Therapy Program
Cancer Treatment Centers of America at Midwestern Regional Medical Center has been recognized by the Foundation for the Accreditation of Cellular Therapy (FACT) at the University of Nebraska Medical Center for demonstrating compliance with the FACT-JACIE International Standards for Cellular Therapy Product Collection, Processing and Administration. [Cancer Treatment Centers of America] Press Release

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Fountain of Youth: A Congressman’s Plan to Make NIH Grantees Younger
A member of Congress has waded into the thorny issue of the graying of U.S. biomedical researchers with a radical solution: He wants to order the National Institutes of Health (NIH) to bring down the average age at which new investigators receive their first grant by four years within a decade. [ScienceInsider] Editorial

End of the Road for Rogue Stem-Cell Therapy in Italy
The Italian health minister Beatrice Lorenzin declared that her government will not support a clinical trial of a controversial stem cell therapy that it promised last year. [Nature News] Editorial

E.U. Medicines Agency Hands Victory to Advocates of Trial Data Sharing
After an 18-month saga, the European Medicines Agency has approved the details of a new system allowing researchers to scrutinize unpublished data from clinical trials. [ScienceInsider] Editorial
NEW CIRM Showcase: Accelerating Stem Cell Treatments to Patients
November 5, 2014
San Francisco, United States

NEW Global Controls in Stem Cells
November 5-7, 2014
Singapore, Singapore

Visit our events page to see a complete list of events in the ESC & iPSC community.
NEW Postdoctoral Fellow – Pluripotent Stem Cell Laboratory (University of California – San Diego)

NEW PhD Position – DNA Demethylation, Reprogramming & Differentiation (Institute of Molecular Biology)

Postdoctoral Fellowship – Stem Cells & Epigenetics (National Institutes of Health)

Postdoctoral Positions – Stem Cells & Regenerative Medicine (Texas Biomedical Research Institute)

Postdoctoral Position – Transplantation Aspects of Hepatocytes Derived from Human Pluripotent Stem Cells (INSERM UMR 1064)

Postdoctoral Fellow – Cardiac Differentiation of Pluripotent Stem Cells (Stanford University)

Postdoctoral Fellow – Cardiac Animal Surgery (Stanford University)

Postdoctoral Positions – Hematopoietic and Induced Pluripotent Stem Cells (Indiana University School of Medicine)

Scientist – Pluripotent Stem Cell Biology Endoderm Lineages (STEMCELL Technologies Inc.)

Scientist – Human Pluripotent Stem Cell Biology (STEMCELL Technologies Inc.)

Scientist – Pluripotent Stem Cell Media Development, High Throughput Screening (STEMCELL Technologies Inc.)

Scientist – Cell Culture Support Products (STEMCELL Technologies Inc.)

Scientist – Immunology/Cell Separation (STEMCELL Technologies Inc.)

Research Associate – Cell Separation (STEMCELL Technologies Inc.)

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