Volume 10.00 | Jan 7

ESC & iPSC News 10.00 January 7, 2015
ESC & iPSC News
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Human Primordial Cells Created in the Lab
Researchers managed the feat of turning back the clock on human cells to create primordial germ cells – the embryonic cells that give rise to sperm and ova – in the lab. This is the first time that human cells have been programmed into this early developmental stage. [Press release from the Weizmann Institute of Science discussing online prepublication in Cell] Press Release | Full Article | Graphical Abstract
Free Wallchart: Small Molecules, Big Impact in PSC Research
PUBLICATIONS (Ranked by impact factor of the journal)
Differentiation of Hypothalamic-Like Neurons from Human Pluripotent Stem Cells
Investigators developed a protocol for efficient generation of hypothalamic neurons from human embryonic stem cells and induced pluripotent stem cells obtained from patients with monogenetic forms of obesity. [J Clin Invest] Full Article

MSCs Derived from iPSCs with a Modified Protocol Are Tumor-Tropic but Have Much Less Potential to Promote Tumors than Bone Marrow MSCs
Researchers derived mesenchymal stem or stromal cells (MSCs) from transgene-free human induced pluripotent stem cells (iPSCs) efficiently with a modified protocol that eliminated the need of flow cytometric sorting. These iPSC-derived MSCs homed to cancers with efficiencies similar to bone marrow (BM)-MSCs but were much less prone than BM-MSCs to promote the epithelial-mesenchymal transition, invasion, stemness, and growth of cancer cells. [Proc Natl Acad Sci USA] Abstract

3D Imaging of Sox2 Enhancer Clusters in Embryonic Stem Cells
Scientists combined lattice light-sheet imaging, single-molecule tracking, numerical simulations, and ChIP-exo mapping to localize and functionally probe Sox2 enhancer-organization in living embryonic stem cells. [eLife] Full Article

Signaling through Retinoic Acid Receptors Is Required for Reprogramming of Both MEFs and EpiSCs to iPSCs
Investigators explored the role of retinoic acid in reprogramming and report that the six factors efficiently and directly reprogram mouse embryonic fibroblast cells (MEFs) into integration-free induced pluripotent stem cells (iPSCs) in defined medium in the absence of feeder cells. [Stem Cells] Abstract | Full Article

Inhibition of TGF-β Signaling Can Substitute for Oct4 in Reprogramming and Maintain Pluripotency
Scientists show that inhibiting TGF-β signaling with its inhibitor, SB431542, can substitute for Oct4 during reprogramming. Moreover, inhibiting TGF-β signaling can sustain the pluripotency of induced pluripotent stem cells and embryonic stem cells through modulating FGF/MEK/ERK signaling. [J Biol Chem]
Abstract | Full Article

An Alternative Retinoic Acid Responsive Stra6 Promoter Regulated in Response to Retinol Deficiency
Researchers show that in embryonic stem (ES) cells and in some tissues the Stra6 gene encodes two distinct mRNAs transcribed from two different promoters. While both are all-trans retinoic acid (RA) responsive in ES cells, the downstream promoter contains a half-site RA response element and drives an approximately 13-fold, RA-associated increase in luciferase reporter activity. [J Biol Chem] Abstract | Full Article

Hepatic Regenerative Potential of Mouse Bone Marrow Very Small Embryonic-Like Stem Cells
The authors report the whole genome gene expression profile of very small embryonic-like stem cells (VSELs) and embryonic stem (ES) cells. They analyzed the global gene expression of VSELs and compared it with ES cells by microarray analysis. [J Cell Physiol] Abstract

Electrospun Biomaterial Scaffolds with Varied Topographies for Neuronal Differentiation of Human-Induced Pluripotent Stem Cells
Scientists investigated the effect of micro and nanoscale scaffold topography on promoting neuronal differentiation of human induced pluripotent stem cells and directing the resulting neuronal outgrowth in an organized manner. [J Biomed Mater Res A] Abstract

Restoration of Progranulin Expression Rescues Cortical Neuron Generation in an Induced Pluripotent Stem Cell Model of Frontotemporal Dementia
To understand how haploinsufficiency of progranulin causes frontotemporal dementia, researchers created induced pluripotent stem cells from patients carrying the GRNIVS1+5G > C mutation. [Stem Cell Reports]
Full Article | Graphical Abstract | Press Release

Successful Reprogramming of Epiblast Stem Cells by Blocking Nuclear Localization of β-Catenin
Investigators describe dramatic improvement of conversion efficiency from primed to naive-like pluripotent stem cells through upregulation of E-cadherin in the presence of the cytokine LIF. [Stem Cell Reports] Full Article

Induce Neural Progenitor Cells Faster and More Effectively - Watch New Technical Video
Retroviral Transcriptional Regulation and Embryonic Stem Cells: War and Peace
Many of the transcription factors in embryonic stem (ES) cells control both host and retroviral genes coordinately, such that retroviral expression patterns can serve as markers of ES pluripotency. [Mol Cell Biol] Abstract

Stem Cell Reprogramming: Basic Implications and Future Perspective for Movement Disorders
Researchers introduce reprogramming strategies and explain the critical steps in the differentiation of human induced pluripotent stem cells to clinical relevant subtypes of cells in the context of movement disorders. [Mov Disord] Abstract

Visit our reviews page to see a complete list of reviews in the ESC & iPSC research field.
Philanthropists John and Leslie Malone Propel Regenerative Medicine with $42.5 Million Gift
Philanthropists John and Leslie Malone have committed a record $42.5 million to Colorado State University to develop regenerative medical therapies for animals and people. [Colorado State University] Press Release

Reprogramming Liver Cells into Pancreas Cells
Diabetes researcher Dr. Francesca Spagnoli has been awarded an extra research grant from the European Research Council. After having identified a factor that converts murine liver cells into pancreas cells, she wants to investigate whether human liver cells can also be reprogrammed into insulin-producing cells. [Max Delbrück Center for Molecular Medicine Berlin-Buch] Press Release

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Questions Linger Over Stress-Induced Stem Cells
The latest investigation into a debunked method of generating stem cells has left researchers grappling with questions about what went wrong in a laboratory at the RIKEN research institute in Japan. The final report from the independent investigation bolstered suspicions that the stem cells — which were supposedly generated by applying stress to ordinary adult cells in an acid bath — were actually embryonic stem cells that had been introduced to the samples. [Nature News] Editorial

India’s Major Science Funders Join Open-Access Push
Two of India’s major science funding agencies are joining the push to make the results of the research they fund freely available to the public. India’s Ministry of Science & Technology announced it will require researchers who receive even just part of their funding from its biotechnology and science and technology departments to deposit copies of their papers in publicly accessible depositories. [ScienceInsider] Editorial
NEW The Biology of Regenerative Medicines
April 22-24, 2015
Cambridge, United Kingdom

Visit our events page to see a complete list of events in the ESC & iPSC community.
NEW Postdoctoral Fellow – Wnt Signaling and Cell Polarity in Embryonic Development (Icahn School of Medicine at Mount Sinai)

NEW Research Technician – Skeletal Muscle Disorders with Human iPS Cells (Johns Hopkins University School of Medicine)

NEW Postdoctoral Fellow – Pluripotent Stem Cells and Cartilage Biology (Boston Children’s Hospital)

NEW PhD Studentship – Deciphering Mechanisms Underlying the Dysfunction of Motoneurons Derived from ALS Patient iPSCs (University of St. Andrews)

Postdoctoral Scientist – Stem Cell Models of Autism (University of South Carolina)

Postdoctoral Fellow – Stem Cell Biology and Reprogramming (Baylor College of Medicine)

Postdoctoral Fellow and Technician – Regenerative Medicine Using iPSC, Embryonic and Adult Stem Cells (University of Illinois at Chicago)

Postdoctoral Position – Pluripotent Stem Cell Biology and Cancer Research (Memorial Sloan-Kettering Cancer Center)

Scientist – Reprogramming and Pluripotent Stem Cell Biology (STEMCELL Technologies Inc.)

Scientist – Pluripotent Stem Cell Biology Endoderm Lineages (STEMCELL Technologies Inc.)

Scientist – Human Pluripotent Stem Cell Biology (STEMCELL Technologies Inc.)

Scientist – Pluripotent Stem Cell Media Development, High Throughput Screening (STEMCELL Technologies Inc.)

Scientist – Cell Culture Support Products (STEMCELL Technologies Inc.)

Scientist – Immunology/Cell Separation (STEMCELL Technologies Inc.)

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