Volume 11.07 | Feb 24

ESC & iPSC News 11.07 February 24, 2016
ESC & iPSC News
     In this issue: Publications | Reviews | Industry News | Policy News | Events | Jobs
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Scientists Find Popular Stem Cell Techniques Safe
Scientists looked at three popular methods of induced pluripotent stem cells (iPSC) production (integrating retroviral vectors, non-integrating Sendai virus and synthetic mRNAs), assessing each for the potential to trigger cancer-causing mutations. While they noted some minor alterations in the iPSCs, none of the methods led to significant mutations. [Press release from The Scripps Research Institute discussing online publication in Nature Communications] Press Release | Full Article
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PUBLICATIONS (Ranked by impact factor of the journal)
A Short G1 Phase Imposes Constitutive Replication Stress and Fork Remodeling in Mouse Embryonic Stem Cells
Researchers showed in cultured embryonic stem cells and mouse embryos that H2AX phosphorylation is dependent on ataxia telangiectasia and Rad3 related and is associated with chromatin loading of the ssDNA-binding proteins RPA and RAD51. [Nat Commun] Full Article

Initiation of Stem Cell Differentiation Involves Cell Cycle-Dependent Regulation of Developmental Genes by Cyclin D
Investigators demonstrated that cell cycle regulators Cyclin D1–3 control cell fate decisions in human pluripotent stem cells by recruiting transcriptional corepressors and coactivator complexes onto neuroectoderm, mesoderm, and endoderm genes. [Genes Dev] Abstract

Aurkb/PP1-Mediated Resetting of Oct4 during the Cell Cycle Determines the Identity of Embryonic Stem Cells
Investigators demonstrated that the regulation of Oct4 by aurora kinase b (Aurkb)/protein phosphatase 1 (PP1) during the cell cycle is important for resetting Oct4 to pluripotency and cell cycle genes in determining the identity of embryonic stem cells. [eLife] Abstract | Download Full Article

NRF2 Orchestrates the Metabolic Shift during Induced Pluripotent Stem Cell Reprogramming
Scientists developed a lentiviral reporter system to assay longitudinal changes in cell signaling and transcription factor activity in living cells throughout induced pluripotent stem cells reprogramming of human dermal fibroblasts. [Cell Rep] Full Article | Graphical Abstract

Enrichment of G2/M Cell Cycle Phase in Human Pluripotent Stem Cells Enhances HDR-Mediated Gene Repair with Customizable Endonucleases
Investigators observed improved efficiency using ZFNs, TALENs, two CRISPR/Cas9, and CRISPR/Cas9 nickase to target five genes in three human pluripotent stem cells lines. [Sci Rep] Full Article

TRPV-1-Mediated Elimination of Residual iPS Cells in Bioengineered Cardiac Cell Sheet Tissues
Researchers showed that TRPV-1 activation through transient culture at 42°C in combination with agonists is a simple and useful strategy to eliminate induced pluripotent stem cells from bioengineered cardiac cell sheet tissues. [Sci Rep] Full Article

SETD7 Regulates the Differentiation of Human Embryonic Stem Cells
The authors identified the methyltransferase SETD7 as highly induced during the differentiation of human embryonic stem cells and differentially expressed between induced pluripotent cells and somatic cells. [PLoS One] Full Article

Mucin-Inspired Thermoresponsive Synthetic Hydrogels Induce Stasis in Human Pluripotent Stem Cells and Human Embryos
Researchers report cellular stasis for both human pluripotent stem cells colonies and preimplantation embryos immersed in a wholly synthetic thermoresponsive gel comprising poly(glycerol monomethacrylate)-poly(2-hydroxypropyl methacrylate) diblock copolymer worms. [ACS Cent Sci] Full Article | Press Release

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Molecular Features of Cellular Reprogramming and Development
The authors discuss the control and maintenance of cellular identity during developmental transitions as they have been studied using direct reprogramming, with an emphasis on transcriptional and epigenetic regulation. [Nat Rev Mol Cell Biol] Abstract

Visit our reviews page to see a complete list of reviews in the ESC & iPSC research field.

ASGCT 2016 Annual Meeting
Cutting Edge Medical Research Ideas Receive £23.2 Million Boost
The Medical Research Council is accelerating innovative medical research with £23.2 million in new funding to take ground-breaking ideas from UK universities into industry and out to patients. [Medical Research Council] Press Release

Lupus Foundation of America Accepting Applications to Expedite Progress in Lupus Research
The Lupus Foundation of America announced 2016 peer-reviewed research funding to study stem cells in lupus. [Lupus Foundation of America] Press Release

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NIH to Review Its Policies on All Nonhuman Primate Research
The National Institutes of Health (NIH) is about to take a closer look at the use of nonhuman primates in all federally funded U.S. research labs. In response to a congressional mandate, the agency will convene a workshop this summer to review the ethical policies and procedures surrounding work on monkeys, baboons, and related animals. [ScienceInsider] Editorial

Senate Nears Final Vote on Obama’s Pick to Lead FDA
After a five-month delay, the US Senate is poised to vote on the nominee to head the US Food and Drug Administration (FDA): cardiologist and clinical-trials expert Robert Califf. [Nature News] Editorial
NEW 18th International Conference on Cell and Stem Cell Engineering (ICCSCE 2016)
September 15-16, 2016
Rome, Italy

Visit our events page to see a complete list of events in the ESC & iPSC community.
NEW Postdoctoral Position – Human Pluripotent Stem Cells, Neurodevelopmental Disorders and Neural Repair (University of Nebraksa Medical Center)

Scientist – Human Pluripotent Stem Cell Neural Differentiation (STEMCELL Technologies Inc.)

Postdoctoral Position – Stem Cell Biology and Leukemogenesis (Fundação Hemocentro de Ribeirão Preto)

Postdoctoral Fellow – Gene Expression Control (University of Birmingham)

Postdoctoral Positions – Immunology, Cancer, and Stem Cells (University of Connecticut)

PhD Position – Developmental Epigenetics (Institute of Molecular Biology)

Postdoctoral Positions – Human Stem Cells (University of Pittsburgh)

Postdoctoral Scientist – Transcription and Epigenetics (Max-Planck Institute for Molecular Genetics)

Research Associate – ES Cell Biology Team (Kymab Ltd)

Postdoctoral Position – Genetics and Genomics of Stem Cells (Albert Einstein College of Medicine of Yeshiva University)

Postdoctoral Fellow – Bioinformatics and Stem Cell Biology (University of Texas)

Postdoctoral Candidate – Embryonic Stem Cells and Chromatin Biology (University of Michigan)

Development Engineer/Scientist – Regenerative Medicine (Centre for Commercialization of Regenerative Medicine)

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