Volume 15.23 | June 17

2020-06-17 | ESC 15.23
ESC & iPSC News by STEMCELL Technologies
Vol. 15.23 – 17 June, 2020
TOP STORY

An In Vitro Model of Early Anteroposterior Organization during Human Development

The authors showed that human ESCs could be used to generate gastruloids—three-dimensional multicellular aggregates that differentiate to form derivatives of the three germ layers organized spatiotemporally, without additional extra-embryonic tissues.
[Nature]

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Checking your cell quality doesn’t have to be difficult - Genetic screening with the hPSC Genetic Analysis Kit
PUBLICATIONSRanked by the impact factor of the journal

Autism-Associated SHANK3 Mutations Impair Maturation of Neuromuscular Junctions and Striated Muscles

Investigators used a combination of patient-derived human iPSCs, Shank3Δ11(−/−) mice, and Phelan-McDermid syndrome muscle biopsies from patients of different ages to analyze the role of SHANK3 on motor unit development.
[Science Translational Medicine]

Full Article

Immunity-and-Matrix-Regulatory Cells Derived from Human Embryonic Stem Cells Safely and Effectively Treat Mouse Lung Injury and Fibrosis

Intravenous delivery of immunity- and matrix-regulatory cells, derived from human ESCs, inhibited both pulmonary inflammation and fibrosis in mouse models of lung injury, and significantly improved the survival rate of the recipient mice in a dose-dependent manner, likely through paracrine regulatory mechanisms.
[Cell Research]

Full Article

High-Throughput Intracellular Biopsy of microRNAs for Dissecting the Temporal Dynamics of Cellular Heterogeneity

Using intracellular biopsy technique (inCell-Biopsy), scientists analyzed the temporal miRNA transcriptome over the differentiation of ESCs. The combinatorial miRNA expression patterns derived by inCell-Biopsy identified emerging cell subpopulations differentiated from ESCs and revealed the dynamic evolution of cellular heterogeneity.
[Science Advances]

Full Article

Impact of α-Synuclein Pathology on Transplanted hESC-Derived Dopaminergic Neurons in a Humanized α-Synuclein Rat Model of PD

Researchers established a humanized transplantation model of Parkinson’s disease that better recapitulates the main disease features, obtained by coinjection of preformed human α-synuclein (α-syn) fibrils and adeno-associated virus expressing human wild-type α-syn unilaterally into the rat substantia nigra.
[Proceedings of the National Academy of Sciences of the United States of America]

Full Article

Critical Roles of Translation Initiation and RNA Uridylation in Endogenous Retroviral Expression and Neural Differentiation in Pluripotent Stem Cells

Scientists generated a conditional knockout of NAT1 in PSCs and used the cells for the functional analyses of NAT1. Their results showed that NAT1 was required for the self-renewal and neural differentiation of primed PSCs.
[Cell Reports]

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Variable Outcomes in Neural Differentiation of Human PSCs Arise from Intrinsic Differences in Developmental Signaling Pathways

The authors focused on a well-established differentiation process to cerebral cortex neural progenitor cells and their neuronal progeny from human PSCs.
[Cell Reports]

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Cryopreserved Human Oocytes and Cord Blood Cells Can Produce Somatic Cell Nuclear Transfer-Derived Pluripotent Stem Cells with a Homozygous HLA Type

Researchers applied a modified derivation method to the PSC line and first obtained human somatic cell nuclear transfer-PSC lines derived from both donated cryopreserved oocytes and cord blood cells with a homozygous human leukocyte antigen type.
[Stem Cell Reports]

Full Article

MicroRNA-181c-5p Promotes the Formation of Insulin-Producing Cells from Human Induced Pluripotent Stem Cells by Targeting smad7 and TGIF2

Investigators differentiated human induced PSCs into insulin-producing cells in a stepwise process that recapitulated pancreas organogenesis and observed that miR-181c-5p continuously accumulated throughout the entire differentiation process.
[Cell Death & Disease]

Full Article

Human Induced Pluripotent Stem Cell Line Banking for the Production of Rare Blood Type Erythrocytes

Mononuclear cells separated from the peripheral blood of O D-positive and rare blood type donors were cultured to produce and expand erythroid progenitors and reprogrammed into iPSCs. A 31-day serum-free, xeno-free erythroid differentiation protocol was used to generate reticulocytes.
[Journal of Translational Medicine]

Full Article

Selective Induction of Human Autonomic Neurons Enables Precise Control of Cardiomyocyte Beating

Neurons were individually induced, facilitating the regulation of the beating rates of hiPSC-derived cardiomyocytes in an antagonistic manner.
[Scientific Reports]

Full Article

R-Loops Coordinate with SOX2 in Regulating Reprogramming to Pluripotency

Investigators profiled R-loops during somatic cell reprogramming and found that dynamic changes in R-loops were essential for reprogramming and occurred before changes in gene expression.
[Genetics]

Full Article

Human MuStem Cell Grafting into Infarcted Rat Heart Attenuates Adverse Tissue Remodeling and Preserves Cardiac Function hMuStem Cells Preserve Function of Infarcted Heart

In dog and human, the authors describe a type of muscle-derived stem cells termed MuStem cells that efficiently promoted repair of injured skeletal muscle. Enhanced survival rate, long-term engraftment, and participation in muscle fiber formation were reported, leading to persistent tissue remodeling and clinical benefits.
[Molecular Therapy-Methods & Clinical Development]

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REVIEWS

Tooth Formation: Are the Hardest Tissues of the Human Body Hard to Regenerate?

Approaches for whole-tooth regeneration utilizing adult stem cells, iPSCs, or tooth germ cells transplantation are emerging as promising alternatives to overcome existing in vitro tissue generation hurdles. The authors discuss the most recent advances in cellular signaling guiding dental tissue genesis.
[International Journal of Molecular Sciences]

AbstractGraphical Abstract

INDUSTRY AND POLICY NEWS

AgeX Therapeutics and Pluristyx Announce Manufacturing, Marketing, and Distribution Agreement to Expand Access to Clinical-Grade Human Pluripotent Stem Cells for Therapeutic Applications

AgeX Therapeutics, Inc. and Pluristyx, Inc. have announced they have entered into a manufacturing, marketing, and distribution agreement through which Pluristyx will undertake these activities on behalf of AgeX with respect to AgeX’s research- and clinical-grade ESI brand human ESCs.
[AgeX Therapeutics Inc.]

Press Release

Huge Open-Access Journal Deal Inked by University of California and Springer Nature

The University of California (UC) system announced it has signed the biggest open-access deal in North America with one of the largest commercial scientific publishers. The agreement with Springer Nature includes a commitment by the publisher to explore making all articles that UC corresponding authors publish in the Nature family of journals immediately free to read on publication starting in 2022.
[ScienceInsider]

Editorial

FEATURED EVENT

From Stem Cells to Human Development

2020-09-08 – 2020-09-11
Virtual

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JOB OPPORTUNITIES

Postdoctoral Fellow – Human Induced Pluripotent Stem Cell-Derived T Cell Process Development

Genentech – South San Francisco, California, United States

Postdoctoral Scholar – Developmental & Stem Cell Biology

University of California – San Francisco, California, United States

Postdoctoral Fellow – Neuroinflammation

Henry Ford Health System – Detroit, Michigan, United States

Postdoctoral Fellow – CRISPR Neurodegenerative Disease Modeling

Wellcome Sanger Institute – Cambridge, United Kingdom

Postdoctoral Position – Cardiovascular Molecular Mechanics

Spanish National Center for Cardiovascular Research – Madrid, Spain

Scientist – CAR Engineering and Protein Sciences

Century Therapeutics – Philadelphia, Pennsylvania, United States

Research Scientist – Stem Cells

Evotec SE – Hamburg, Germany

Fellowship Position – Genome Stem Cell Research

Center for Biologics Evaluation and Research – Silver Spring, Maryland, United States

Postdoctoral Position – Human iPS Models

The Jackson Laboratory – Ben Harbor, Maine, United States

Postdoctoral Fellow – Bioinformatics of Stem Cell Epigenetics

Albert Einstein College of Medicine – New York, New York, United States

Research Specialist – Stem Cells for Regenerative Medicine

University of Illinois at Chicago – Chicago, Illinois, United States

Principal Scientist – Human iPSC Gene Engineering

Empyrean Therapeutics Ltd – Cambridge, United Kingdom

Postdoctoral Position – Human Pluripotent Stem Cells to Hepatobiliary Tissues

Johns Hopkins University School of Medicine – Baltimore, Maryland, United States

> See All Jobs

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