Volume 9.24 | Jun 25

ESC & iPSC News 9.24 June 25, 2014
ESC & iPSC News
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Chemically Defined Generation of Human Cardiomyocytes
Using human induced pluripotent stem cells derived under chemically defined conditions on synthetic matrices, scientists systematically developed an optimized cardiac differentiation strategy, using a chemically defined medium consisting of just three components: the basal medium RPMI 1640, L-ascorbic acid 2-phosphate and rice-derived recombinant human albumin. [Nat Methods] Abstract | Press Release
Efficiently Expand hPSC-Derived NPCs Without Loss of Multipotency: STEMdiff™ Neural Progenitor Medium
PUBLICATIONS (Ranked by impact factor of the journal)
Notch Inhibition Allows Oncogene-Independent Generation of iPS Cells
Scientists show that inhibition of Notch greatly improves the efficiency of induced pluripotent stem (iPS) cell generation from mouse and human keratinocytes by suppressing p21 in a p53-independent manner and thereby enriching for undifferentiated cells capable of long-term self-renewal. [Nat Chem Biol] Abstract

Evolutionally Dynamic L1 Regulation in Embryonic Stem Cells
Scientists demonstrate that in human embryonic stem cells, KAP1 (KRAB [Krüppel-associated box domain]-associated protein 1), the master cofactor of KRAB-containing zinc finger proteins previously implicated in the restriction of endogenous retroviruses, represses a discrete subset of long interspersed element-1 (L1) lineages predicted to have entered the ancestral genome between 26.8 million and 7.6 million years ago. [Genes Dev] Abstract

Generation of Multipotent Early Lymphoid Progenitors from Human Embryonic Stem Cells
Using a two-step culture procedure investigators identified two original populations of embryonic stem cell-derived hematopoietic progenitor cells with CD34+CD45RA+CD7 and CD34+CD45RA+CD7+ phenotypes. [Stem Cells Dev] Abstract

Drp1 Is Dispensable for Mitochondria Biogenesis in Induction to Pluripotency but Required for Differentiation of Embryonic Stem Cells
The authors tested the role of Dynamin-related protein 1 (Drp1) in the induction and maintenance of pluripotency. They showed that Drp1 band shift occurs in ES cells and iPS cells induced from fibroblasts, in association with mitochondrial morphogenesis. [Stem Cells Dev] Abstract

Genome-Wide Mapping of miRNAs Expressed in Embryonic Stem Cells and Pluripotent Stem Cells Generated by Different Reprogramming Strategies
Scientists demonstrate that reprogrammed cells (induced pluripotent stem cells and nuclear transfer embryonic stem cells [ESCs]) have unique microRNA (miRNA) expression patterns compared with ESCs. [BMC Genomics] Abstract | Full Article

Mechanism of SB431542 in Inhibiting Mouse Embryonic Stem Cell Differentiation
Considering that SB431542 (SB) could not maintain embryonic stem cells pluripotency when leukemia inhibitory factor was withdrawn, scientists sought to identify the mechanism of SB on pluripotent maintenance. [Cell Signal] Abstract

High Efficiency Differentiation of Functional Hepatocytes from Porcine Induced Pluripotent Stem Cells
Researchers generated induced pluripotent stem cells (iPSCs) from porcine ear fibroblasts by overexpressing Sox2, Klf4, Oct4, and c-Myc, and developed a novel strategy for the efficient differentiation of hepatocyte-like cells from porcine iPSCs by following the processes of early liver development. [PLoS One] Full Article

Generation of the SCN1A Epilepsy Mutation in hiPS Cells Using the TALEN Technique
Combining the Transcription Activator-Like Effector Nuclease (TALEN) and induced pluripotent stem (iPS) cell methods, scientists developed two iPS cell lines by generating the point mutation A5768G in the SCN1A gene, which encodes the voltage-gated sodium channel Nav1.1 α subunit. [Sci Rep] Full Article

CCL2 Enhances Pluripotency of Human Induced Pluripotent Stem Cells by Activating Hypoxia Related Genes
Scientists show that chemokine ligand 2 (CCL2) enhances the expression of pluripotent marker genes through the phosphorylation of the signal transducer and activator of transcription 3 protein. [Sci Rep]
Full Article | Press Release

A Method to Identify and Isolate Pluripotent Human Stem Cells and Mouse Epiblast Stem Cells Using Lipid Body-Associated Retinyl Ester Fluorescence
Researchers describe the use of a characteristic blue fluorescence to identify and isolate pluripotent human embryonic stem cells and human-induced pluripotent stem cells. [Stem Cell Rep]
Full Article | Graphical Abstract

New TeSR™-E8™ is Here for Feeder-Free Culture of Human ES Cells and iPS Cells
Human Stem Cell Models of Dementia
Positive predictions were made in the aftermath of the development of induced pluripotent stem cell (iPSC) technology for the use of patient-specific iPSCs to model neurological diseases, including dementia. The author reviews the current state of the field, and explores how close we are to the goal of in vitro models that capture all aspects of the cell and molecular biology of dementia. [Hum Mol Genet] Abstract | Full Article

Visit our reviews page to see a complete list of reviews in the ESC & iPSC research field.

BioProcess International Conference & Exhibition - Boston MA
NYSCF and eagle-i Network Co-Develop iPS Cell Database
Recognizing the research potential of shared induced pluripotent stem (iPS) cell lines, the New York Stem Cell Foundation (NYSCF) Research Institute and the eagle-i Network will make NYSCF iPS cell lines and related information available to the public on a user-friendly, web-based, searchable database. [The New York Stem Cell Foundation] Press Release

Yale Stem Cell Researchers Receive $5.6 Million in State Grants
Yale researchers received a total of $5.6 million for stem cell research out of $10 million given to 18 state-based researchers. The largest sum, $1.2 million, is a group grant given to Yale University, led by principal investigator Joseph Craft, for research into the therapeutic role of induced pluripotent stem cells in human lupus, an autoimmune disease. [The New Haven Register] Article

From our sponsor: Free wallchart from Nature Neuroscience – Neural Stem Cells. Request your copy.
USC Considers Acquiring Scripps Research Institute
In an unexpected announcement, the University of Southern California (USC) in Los Angeles revealed it may be acquiring or merging with the Scripps Research Institute. Scripps issued a joint statement with the university, telling that they were “discussing the possibility of a relationship that would enhance the missions of both institutions.” [ScienceInsider] Editorial

Stem Cells: Taking a Stand against Pseudoscience
Elena Cattaneo and Gilberto Corbellini are among the academics working to protect patients from questionable stem-cell therapies. They share their experiences and opinions of the long, hard fight for evidence to prevail. [Nature News] Editorial
NEW NYSCF NY Stem Cell Foundation 9th Annual Gala and Science Fair
October 14, 2014
New York City, United States

Visit our events page to see a complete list of events in the ESC & iPSC community.
NEW Induced Pluripotent Stem Cell Technician (JAM Scientific and Medical)

Postdoctoral Positions – Stem Cell Biology (State University of New York)

Postdoctoral Position – Studying RNA and Heart Biology Using Human Stem Cells (University of Pittsburgh)

Postdoctoral Position – Functional Characterization of Neurons Derived from Human Induced Pluripotent Stem Cells (Radboud University Medical Center)

Postdoctoral Researcher – Hematopoietic Stem Cells as Well as on Induced Pluripotent Stem Cells (KU Leuven)

Postdoctoral Position – Epigenetics and Stem Cell Biology (Max Planck Institute for Heart and Lung Research)

PhD Studentship – Molecular Mechanism of Asymmetric Stem Cell Division (King’s College London)

Postdoctoral Fellows – Reproductive Sciences/Embryonic Development (University of Maryland)

Postdoctoral Position – Epigenetics, Stem Cell Biology and Heart Development (Max Planck Institute for Heart and Lung Research)

Postdoctoral Associate – Hematopoietic Differentiation (Northwestern University Feinberg School of Medicine)

Scientist – Pluripotent Stem Cell Biology Endoderm Lineages (STEMCELL Technologies Inc.)

Scientist – Human Pluripotent Stem Cell Biology (STEMCELL Technologies Inc.)

Scientist – Pluripotent Stem Cell Media Development, High Throughput Screening (STEMCELL Technologies Inc.)

Scientist – Cell Culture Support Products (STEMCELL Technologies Inc.)

Scientist – Immunology/Cell Separation (STEMCELL Technologies Inc.)

Research Associate – Cell Separation (STEMCELL Technologies Inc.)

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